Studies on Application of Ion Associate to Amperometric Biosensors for Glucose Based on Paste Modified by FeCp2

Abstract

Abstract: This paper describes the design and testing of amperometric glucose biosensor in whichelectrical communication between the flavin redox centers of glucose oxidase and a conventionalcarbon paste electrode containing (C₆H₅).1B · N⁺(CH₃)₁ has been achieved by using electron-transfer relay system based on ferrocene (FeCp₂).Characteristics of the biosensor has been shown by cyclic voltammetry and stationary potential measurements.The biosensor responds rapidly to glucose, with steady-state current responsed achieved in less than 50 s.The response to glucose under N₂ saturation showed apparent Michcells-Menten constant, K_M^app, 21 mmol/L.The biosensor can be operated in a potential range where the oxidation of common interfering species, such as V_c and uric acid does not occur.Life of the biosensor is 50 days and plasma glucose assays were correlated with spectropllotometric determinations(Y=10.04 + 0.886X, r=0.99, n=9).

Key words: Ferrocene, Ion associate, Biosensor, Glucose

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SUN Chang-Qing, SONG Wen-Be, GAO Qian, DU Wen-Yuan, XU Hong-Ding . Studies on Application of Ion Associate to Amperometric Biosensors for Glucose Based on Paste Modified by FeCp₂[J]. Chem. J. Chinese Universities, 1993, 14(8): 1073.

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Studies on Application of Ion Associate to Amperometric Biosensors for Glucose Based on Paste Modified by FeCp₂

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