Studies on the Immobilization of π-Amylase by Radiation Polymerization

Abstract

Abstract: The present paper covers the immobilization of α-amylase onto PHEMA by radiation polymerization at low temperatures.The proper condition of the radiation polymerization was mainly investigated.The relative activity of transforming amylogen into reduced sugar and the stability of repeated application of immobilized enzymes obtained from different systems are discussed.The paper narrates the relationship between the relative activity and microhole of the polymer matrix as well.The experiments show that the best conditions are as follows: [E]≥250μg/mL,[M] = 30%～40%, T=-50～55℃, R=5.6×10⁵ rad, vacuum system.The rela-tive activity of the immobilized enzyme got under the above conditions keeps higher than 30% even after being used for more than ten times

Key words: Radiation polymerization, Immobilization, a-Amylase

TrendMD:

YAN Wen-li, FENG Zhen-tai, CHEN Guo-hua, LIU Yi-ling . Studies on the Immobilization of π-Amylase by Radiation Polymerization[J]. Chem. J. Chinese Universities, 1992, 13(4): 554.

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Studies on the Immobilization of π-Amylase by Radiation Polymerization

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