Application and Current Response of Electrochemical and Immobile Polypyrrole Cholesterol Oxidase Electrode

Abstract

Abstract: This article reports a new kind of PP/GOX/cholesterol oxidasemodifidd electrode. In the presence of enzyme, pyrrole was electrolysed and precipitated, its monomer was electrochemically oxidized and polymerized to form membrane which carries positive charge. In the process of membrane formation, the anion entered the membrane. After studying the electropolymerizing conditions, the electrochemical behaviour and other properties of this electrode, we found that in order to improve measuring sensitivity and accuration and reduce working potential and diminish background current, Mo⁶⁺should be added to the solution, the catalyst I^-is oxidized by H₂O₂to form I₂, then, the existence of cholesterol can be determined by measuring the reduced current of I₂.This method can be applied to measuring tolly cholesteol and the result is satisfactory. Manufacturing this kind of electrodes is significant for modified electrode miniaturization and living body analysis.

Key words: Polypyrrole, Electropolymerizing, Cholesterol oxidase electrode, Total cholesterol

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Lou Yin-hua, Zheng Dong-sheng, Zhang Rong-kun, Wang Jin. Application and Current Response of Electrochemical and Immobile Polypyrrole Cholesterol Oxidase Electrode[J]. Chem. J. Chinese Universities, 1991, 12(10): 1320.

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Application and Current Response of Electrochemical and Immobile Polypyrrole Cholesterol Oxidase Electrode

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