Abstract: This paper describes the preparation of the glassy carbon electrode treated with Nafion-130 containing Schiff base and the process of electrochemical response. It was proved that mercury ions can be complexed with Schiff base, which is verified by IRspectral analysis. The Nafion-Schiff base coated electrode was used to determine trace mercury, and improved the resistance to interference from surface active compounds and increased sensitity. The result was satisfactory when the NSCME was used to determine trace mercury in untreated urine sample.

Key words: Nafion, Schif f base, Stripping voltammetry, Chemically modified electrode, Mercury