Studies on the Structure of the Alkaline Proteinase BP

Abstract

Abstract: The amino-acid composition of the alkaline proteinase BP was determined by amino-acid analyzer. The molecular weight of the alkaline proteinase BP measured by gel filtration is 19500. The enzyme was modified by reagent NBS and two residues of tryptophan were determined by ultraviolet spectrometry. One of them is the necessary residue for the activity of the enzyme. The two tryptophan residues were located on the surface of the alkaline proteinase BP, which had been determined by the reagents of the surface fluorescence quench. The conformation of the enzyme molecule is a random coil proved by the CD spectrum.

Key words: Alkaline proteinase BP, Amino-acid composition, Molecular weight, Tryptophan residue, CD spectrum

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Liu Lanying, Feng Yan, Zhang Lifang, Ma Rongjiu, Cheng Yuhua. Studies on the Structure of the Alkaline Proteinase BP[J]. Chem. J. Chinese Universities, 1990, 11(10): 1116.

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Studies on the Structure of the Alkaline Proteinase BP

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