Vinyl Sulfone Functionalized Magnetic Nanoparticles and Its Application in Palmitoylated Peptides Enrichment

doi:10.7503/cjcu20230239

Abstract

Abstract:

Vinyl sulfone modified magnetic nanoparticles（denoted as MagBVP） were synthesized and used as the magnetic carrier of magnetic solid phase extraction（MSPE） platform， combined with MALDI-TOF MS or LC-MS/MS. The MagBVP was characterized by means of vibrating sample magnetometer， field emission scanning electron microscopy， infrared spectroscopy， zeta potential measurement and X-ray diffraction. The synthesized palmitoylated palmitoyl-coA was selected as the modeling palmitoylated peptide sample. Under the optimal conditions， the MSPE-MS platform could achieve high selectivity（BSA tryptic digests/palmitoylated peptide molar ratio of 2000∶1） and sensitivity（the detection limit was determined to be as low as 1.0 fmol/μL） for palmitoylated peptide. Finally， the method was applied to the determination of palmitoylated peptides in cell lysates samples. These results showed that the magnetic material modified by vinyl sulfone had a high selectivity for palmitoylomics.

Key words: Sulfone, Magnetic nanoparticle, Magnetic solid phase extraction, Palmitoylomics, Mass spectrometry

CLC Number:

O652.6

TrendMD:

ZHENG Haijiao, XU Wenhui, JIA Qiong. Vinyl Sulfone Functionalized Magnetic Nanoparticles and Its Application in Palmitoylated Peptides Enrichment[J]. Chem. J. Chinese Universities, 2023, 44(11): 20230239.

Figures/Tables 7

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