Differentiation and Investigation of Ginsenoside Isomers 20（S）-Rf and Rg1 by Energy-resolved Mass Spectrometry

doi:10.7503/cjcu20220576

Abstract

Abstract:

An energy-resolved mass spectrometry（ERMS） method was developed for the differentiation and investigation of ginsenoside isomers 20（S）-Rf and Rg₁ by combining continuously varied collision energy with tandem mass spectrometry. The ratio of product ion intensity to its precursor ion intensity was defined as intensity fraction（IF）， whereas the ratio of two product ion intensities was defined as intensity ratio（IR）. IF and IR were plotted against the continuously varied collision energy to establish the IF and IR curves， respectively. Although the tandem mass spectra of ginsenoside isomers 20（S）-Rf and Rg₁ were almost identical， multivariate statistical analysis showed significant differences in the IF and IR profiles of their characteristic fragment ions ［M-Glc-H］^‒，［M-2Glc-H］^‒， and ［M-2Glc-C₆H₁₂-H］^‒ with distinct differentiation boundaries that could directly distinguish 20（S）-Rf and Rg₁. The relative molar contents of 20（S）-Rf and Rg₁ in the biotransformation products of protopanaxtriol-type ginsenosides were further analyzed in real time using ERMS. All the results showed that ERMS enables the rapid differentiation and relative content monitoring of 20（S）-Rf and Rg₁ without the separation by liquid chromatography.

Key words: Energy-resolved mass spectrometry, Structural isomer, Ginsenoside 20（S）-Rf, Ginsenoside Rg₁, Relative quantification analysis

CLC Number:

O657

TrendMD:

ZHAO Huanxi, LI Zhuo, ZHAO Mengya, TIAN Lu, XIAO Yusheng, WANG Zhenhuan, YUE Hao, XIU Yang. Differentiation and Investigation of Ginsenoside Isomers 20（S）-Rf and Rg₁ by Energy-resolved Mass Spectrometry[J]. Chem. J. Chinese Universities, 2023, 44(4): 20220576.

Figures/Tables 9

References 22

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Product ion	IF	A₀	A₁	A₂	A₃	A₄	A₅
m/z 637.5	0.032	0.026	0.012	0.023	0.046	0.016	0.021
m/z 475.5	0.045	0.032	0.028	0.017	0.038	0.035	0.042
m/z 391.4	0.017	0.002	0.008	0.006	0.002	0.004	0.006

Product ion	IF	A₀	A₁	A₂	A₃	A₄	A₅
m/z 637.5	0.032	0.026	0.012	0.023	0.046	0.016	0.021
m/z 475.5	0.045	0.032	0.028	0.017	0.038	0.035	0.042
m/z 391.4	0.017	0.002	0.008	0.006	0.002	0.004	0.006

Product ion pair	IR	A₀	A₁	A₂	A₃	A₄	A₅
m/z 637.5 to m/z 475.5	0.002	0.004	0.002	0.006	0.007	0.006	0.008
m/z 637.5 to m/z 391.4	0.107	0.009	0.016	0.089	0.216	0.328	0.671
m/z 475.5 to m/z 391.4	0.086	0.008	0.039	0.012	0.067	0.107	0.164

Product ion pair	IR	A₀	A₁	A₂	A₃	A₄	A₅
m/z 637.5 to m/z 475.5	0.002	0.004	0.002	0.006	0.007	0.006	0.008
m/z 637.5 to m/z 391.4	0.107	0.009	0.016	0.089	0.216	0.328	0.671
m/z 475.5 to m/z 391.4	0.086	0.008	0.039	0.012	0.067	0.107	0.164

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Differentiation and Investigation of Ginsenoside Isomers 20（S）-Rf and Rg₁ by Energy-resolved Mass Spectrometry

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