Ratiometric Fluorescence Assay for Visual Detection of Tetracycline Residues based on the Complex of Carbon Dots and Europium †

doi:10.7503/cjcu20190379

Abstract

Abstract:

On combination of the fluorescence quenching effect of tetracycline on carbon dots(CDs) with the fluorescence enhancement effect of tetracycline on europium ions(Eu ³⁺), a visual ratiometric fluorescence assay was developed for the convenient detection of tetracycline residues. By optimizing the CDs/Eu ³⁺ ratio, pH and other experimental conditions, the detection system exhibited a distinct chang of fluorescence color(blue→pink→red) in response to tetracycline, leading to a convenient identification and semi-quantitation by the naked eye. The results showed that the logarithmic values of I₆₁₈/I₄₄₀ was linearly related to the concentration of tetracycline in the range of 20—100 nmol/L, and the detection limit of this work was 1 nmol/L. Recovery experiment suggested the proposed method fine results for the determination of tetracycline in water samples.

Key words: Carbondots, Europium, Ratiometric fluorescence assay, Visual detection, Tetracycline

CLC Number:

O657.3

TrendMD:

YANG Weiqiang, ZHANG Guiyun, LIN Hua, NI Jiancong, HUANG Lingfeng. Ratiometric Fluorescence Assay for Visual Detection of Tetracycline Residues based on the Complex of Carbon Dots and Europium ^†[J]. Chem. J. Chinese Universities, 2019, 40(11): 2294.

Figures/Tables 7

Fig.1 Fluorescence quenching effect of tetracycline on CDs(A) and fluorescence enhancement effect of tetracycline on Eu3+ (B) c(Tetracycline)/(nmol·L-1): a. 0; b. 20; c. 40; d. 60; e. 80; f. 100. ρ(CDs)=1 μg/mL, c(Eu3+)=20 μmol/L. The NaOH-H3BO3 buffer solution concentration was 20 mmol/L with pH=9.0. Insets are the linear relationships between the fluorescence intensities of CDs(A) and Eu-TC(B) and the concentration of tetracycline, respectively.

Fig.2 Schematic diagram for the visual detection of tetracycline residues based on the ratiometric fluorescent probe of CDs-Eu3+

Fig.3 Effect of mass concentration ratio of CDs/Eu3+ on the fluorescence assay(A) and effect of pH on the fluorescence of CDs and Eu-TC(B) (A) c(Tetracycline)=50 nmol/L, ρ(CDs)=1—3 μg/mL, c(Eu3+)=6—60 μmol/L. The NaOH-H3BO3 buffer solution concentration was 20 mmol/L(pH=9.0); (B) c(tetracycline)=100 nmol/L, ρ(CDs)=1 μg/mL, c(Eu3+)=20 μmol/L. The NaOH-H3BO3 buffer solution concentration was 20 mmol/L.

Fig.4 Fluorescence spectrum of CDs-Eu3+ probe varying with the concentration of tetracycline(A) and linear relationship between the lg(I618/I440) and the concentration of tetracycline(B) (A) The inset was the fluorescence color change of the solution. c(Tetracycline)/(nmol·L-1): a. 0; b. 20; c. 40; d. 60; e. 80; f. 100. ρ(CDs)=1 μg/mL; ρ(Eu3+)=20 μmol/L. The NaOH-H3BO3 buffer solution concentration was 20 mmol/L with pH=9.0.

Fig.5 Fluorescence response of CDs-Eu3+ probe to different antibiotics The concentration of tetracycline(TC), oxytetracycline(OTC), chlorotetracycline(CTC) and doxycycline(DOX) were 100 nmol/L; and the concentration of penicillin(PCN), erythromycin(ERY), ciprofloxacin(CIP), sulfadiazine(SD) and chloramphenicol(CAP) were 1 μmol/L.

Interference	Concentration of interference/(μmol·L^-1)	Error(%)	Interference	Concentration of interference/(μmol·L^-1)	Error(%)
LAS	50.0	3.58	Al³⁺	5.0	-2.67
CPC	50.0	2.89	Fe³⁺	5.0	3.16
Tween-80	20.0	3.77	Cu²⁺	10.0	2.53
Ca²⁺	50.0	-0.83	Zn²⁺	50.0	1.12
Mg²⁺	50.0	-0.15	Pb²⁺	20.0	0.45

Sample	Add/(nmol·L^-1)	Found^*/(nmol·L^-1)	Recovery(%)	RSD(%, n=3)
1	5.0	5.33	106.6	4.7
2	20.0	20.33	101.7	3.9
3	80.0	78.21	97.8	2.5

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