Rapid Detection on Chlortetracycline Residues in Honey by Surface-enhanced Raman Spectroscopy†

doi:10.7503/cjcu20180016

Abstract

Abstract:

The surface-enhanced Raman spectroscopy(SERS) technique was used to rapidly and non-destructively detect the residues of chlortetracycline(CTC) in honey. The prepared gold nanoparticles were used as Raman signal enhancement module. The influence of volume ratio and interact time between gold nanoparticles and CTC was discussed and optimized. Different concentrations of CTC solution were detected. The linear equation of concentration and Raman signal strength on characteristic peak was drawing. After pretreated, the recovery rate of honey sample was detected by the recovery of standard addition. The results show that the characteristic peak of chlortetracycline in 1450 cm^-1 could be used to detecting chlortetracycline(CTC) residues in honey. The best volume ratio of gold nanoparticle to CTC was 1:1 and the best interact time was 1 min. Within the scope of 10—250 mg/L, there was a favorable linearity between the concentration of CTC and SERS characteristic peak in 1450 cm^-1. Linear equation was Y=1×10^-4X+0.0110, and the coefficient of determination was 0.9948. Recovery rate was from 80% to 120% when detecting chlortetracycline(CTC) residues in honey. This detection method is simple, fast, and accurate which provides a new method for detecting antibiotic residues in foods.

Key words: Surface-enhanced Raman scattering(SERS), Au nanoparticle, Chlortetracycline(CTC), Honey

CLC Number:

O657.3

TrendMD:

ZHANG Lutao, ZHOU Guangming, LUO Dan, CHEN Rong. Rapid Detection on Chlortetracycline Residues in Honey by Surface-enhanced Raman Spectroscopy^†[J]. Chem. J. Chinese Universities, 2018, 39(8): 1662.

Figures/Tables 9

Fig.1 UV-Vis absorption spectra of Au NPs(a) and Au NPs-CTC(b)

Fig.2 SEM images of Au NPs(A) and Au NPs-CTC solution(B)

Fig.3 Raman spectra of the solid CTC(a) and the SERS(b) and RRS(c) of the CTC solution

Fig.4 SERS of CTC with different volume ratio of CTC to Au NPsa. 1:1; b. 2:1; c. 1:2; d. 1:3; e. 3:1.

Fig.5 SERS of CTC with different mix duration between CTC and Au NPs(A) and variation of SERS standard peak intensity along with increasing mixing time of CTC and Au NPs(B)

Fig.6 SERS of CTC solution with different concentrationsc(CTC)/(mg·L-1): a. 10; b. 50; c. 100;d. 150; e. 200; f. 250.

Table 1 Comparison of parameters and linear quations at diffferent Raman shift

Raman shift/ cm^-1	Linear equation	R²	SE
745	Y=1×10^-4X+0.0070	0.9868	1.189×10^-3
849	Y=1×10^-4X+0.0063	0.9824	1.337×10^-3
935	Y=1×10^-4X+0.0073	0.9878	1.354×10^-3
1002	Y=9×10^-5X+0.0067	0.9890	1.125×10^-3
1450	Y=1×10^-4X+0.0110	0.9948	1.035×10^-3

Fig.7 Liner regression curve about change of SERS signal of CTC in characteristic peak along with concentration of CTC

Table 2 Recovery experiment of two different kinds of honey

Sample	No.	Added concentration of CTC/ (mg·L^-1)	Detection of CTC concentration/ (mg·L^-1)	Recovery rate(%)
Honey(homemade)	1	0	0	—
	2	0.50	0.476—0.519	95—103
	3	1	0.896—0.997	89—99
Honey(purchased)	1	0	0	—
	2	0.50	0.459—0.495	91—99
	3	1	0.957—1.055	95—105

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