Antifungal Activity and Mechanism of an Essential Oil from Eremothecium ashbyii†

doi:10.7503/cjcu20180477

Abstract

Abstract:

The essential oil was obtained from the broth of Eremothecium ashbyii H4565 by solvent extraction and steam distillation. The chemical components of essential oil were investigated by gas chromatography-mass(GC-MS) spectrometry. Antifungal activities were studied by inhibition zone method. Two fold dilution method was used to determine the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC). Cytoplasmic membrane permeability, protein and potassium ion leakage, and transmission electron microscope were used to determine antifungal mechanism. The results showed that the yield of essential oil was 0.54 g/L. Twenty two compounds, about 98.82% constituents of the total, were identified by GC-MS analysis. The essential oil was composed mainly of monoterpenes, sesquiterpenes, aromatic alcohol, and sesquiterpene hydrocarbons. The essential oil exhibited strong antibacterial activities against C. albicans, C. guilliermondii, C. tropicalis, C. glabrata, C. neoformans and S. cerevisiae. The MIC of the essential oil against these microorganisms were 31.25, 31.25, 62.5, 31.25, 15.625 and 15.625 μg/mL, respectively. The MBC of the essential oil against these microorganisms were 62.5, 62.5, 125.0, 62.5, 31.25 and 31.25 μg/mL, respectively. Based on analyses of cytoplasmic membrane permeability, protein and potassium ion leakage and transmission electron microscope, the possible targets of the essential oil were cell wall and cytoplasmic membrane. The results showed a new light on development and application of the essential oil from microorganism.

Key words: Eremothecium ashbyii, Essential oil, Antifungal activity, Antibacterial mechanism

CLC Number:

O621
Q599

TrendMD:

HE Feng,BAI Jinhai,CHEN Shuxian,TAN Xiaobei. Antifungal Activity and Mechanism of an Essential Oil from Eremothecium ashbyii^†[J]. Chem. J. Chinese Universities, 2019, 40(2): 272.

Figures/Tables 6

References 24

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Compd.	RI^a	RI range^b	CAS number	Content(%)
Limonene	1032	1019—1039	005989-27-5	0.23
Linalool oxide	1077	1061—1090	034995-77-2	0.13
Linalool	1081	1088—1109	000078-70-6	5.30
Phenethyl alcohol	1117	1100—1129	000060-12-8	5.39
Menthone	1153	1125—1174	010458-14-7	0.15
α-Terpineol	1192	1178—1203	010482-56-1	0.42
Citronellol	1234	1215—1237	000106-22-9	34.43
Geraniol	1257	1238—1269	000106-24-1	36.58
Linalyl acetate	1258	1238—1268	000115-95-7	0.19
Geranial	1271	1252—1291	000141-27-5	1.32
Longipinene	1354	1337—1362	005989-08-2	0.47
Eugenol	1359	1345—1375	000097-53-0	0.12
Longicycline	1378	1370—1394	001137-12-8	0.40
β-Elemene	1392	1374—1402	000515-13-9	1.01
Longifolene	1409	1387—1434	000475-20-7	4.22
1-Caryophyllene	1423	1392—1426	000087-44-5	1.77
Thujopsene	1434	1424—1450	000470-40-6	1.11
α-Humulene	1456	1435—1470	006753-98-6	0.32
Germacrene D	1483	1464—1493	023986-74-5	0.34
α-Muurolene	1501	1480—1509	010208-80-7	0.25
Cuparene	1509	1498—1523	016982-00-6	0.11
Nerolidol	1562	1539—1570	007212-44-4	4.56

Compd.	RI^a	RI range^b	CAS number	Content(%)
Limonene	1032	1019—1039	005989-27-5	0.23
Linalool oxide	1077	1061—1090	034995-77-2	0.13
Linalool	1081	1088—1109	000078-70-6	5.30
Phenethyl alcohol	1117	1100—1129	000060-12-8	5.39
Menthone	1153	1125—1174	010458-14-7	0.15
α-Terpineol	1192	1178—1203	010482-56-1	0.42
Citronellol	1234	1215—1237	000106-22-9	34.43
Geraniol	1257	1238—1269	000106-24-1	36.58
Linalyl acetate	1258	1238—1268	000115-95-7	0.19
Geranial	1271	1252—1291	000141-27-5	1.32
Longipinene	1354	1337—1362	005989-08-2	0.47
Eugenol	1359	1345—1375	000097-53-0	0.12
Longicycline	1378	1370—1394	001137-12-8	0.40
β-Elemene	1392	1374—1402	000515-13-9	1.01
Longifolene	1409	1387—1434	000475-20-7	4.22
1-Caryophyllene	1423	1392—1426	000087-44-5	1.77
Thujopsene	1434	1424—1450	000470-40-6	1.11
α-Humulene	1456	1435—1470	006753-98-6	0.32
Germacrene D	1483	1464—1493	023986-74-5	0.34
α-Muurolene	1501	1480—1509	010208-80-7	0.25
Cuparene	1509	1498—1523	016982-00-6	0.11
Nerolidol	1562	1539—1570	007212-44-4	4.56

Microorganism	Diameters of inhibition zone/mm			MIC/ (μg·mL^-1)	MBC/ (μg·mL^-1)
Microorganism	Essential oil	Amphotericin B	n-Hexane	MIC/ (μg·mL^-1)	MBC/ (μg·mL^-1)
C. albicans	28.2±0.3	27.6±0.4	3.1±0.2	31.25	62.5
C. glabrata	28.9±0.5	29.3±0.3	5.2±0.3	31.25	62.5
C. tropicalis	26.1±0.3	26.9±0.2	5.3±0.2	62.5	125.0
C. guilliermondii	27.8±0.2	28.4±0.4	4.2±0.1	31.25	62.5
C. neoformans	31.2±0.2	35.1±0.1	4.4±0.2	15.625	31.25
S. cerevisiae	32.6±0.4	30.4±0.3	4.1±0.3	15.625	31.25

Microorganism	Diameters of inhibition zone/mm			MIC/ (μg·mL^-1)	MBC/ (μg·mL^-1)
Microorganism	Essential oil	Amphotericin B	n-Hexane	MIC/ (μg·mL^-1)	MBC/ (μg·mL^-1)
C. albicans	28.2±0.3	27.6±0.4	3.1±0.2	31.25	62.5
C. glabrata	28.9±0.5	29.3±0.3	5.2±0.3	31.25	62.5
C. tropicalis	26.1±0.3	26.9±0.2	5.3±0.2	62.5	125.0
C. guilliermondii	27.8±0.2	28.4±0.4	4.2±0.1	31.25	62.5
C. neoformans	31.2±0.2	35.1±0.1	4.4±0.2	15.625	31.25
S. cerevisiae	32.6±0.4	30.4±0.3	4.1±0.3	15.625	31.25