Polyethylene Glycol Chemically Modified Soy Protein Isolate Hydrogel†

doi:10.7503/cjcu20170449

Abstract

Abstract:

Soy protein isolate(SPI), a kind of plant protein, has attracted comprehensive interests in many fields due to its sustainability, low cost, functionality, biocompatibility and tunable degradability. However, SPI needs to take an appropriate modification to meet the requirement of practical use because of the weak intermolecular interactions among the polypeptide chains due to its globular protein nature. Therefore, we designed a chemical modification method to change the aggregation state of SPI molecular chains and prepared a SPI-based hydrogel thereafter. Firstly, polyethylene glycol monomethyl ether(mPEG-CHO) was synthesized by Tempo-BAIB method, achieving an average oxidation degree of 56%. Then, mPEG was successfully grafted onto SPI through Schiff’s base reaction between mPEG-CHO and the amino groups in SPI molecular chains, followed by a reduction reaction from NaCNBH₄. It was found that such a PEG modified SPI solution(10%) was able to form a hydrogel at 37 ℃ spontaneously. In the meantime, it showed that with the increase in grafting ratio, the gelation time can be within 30 min. Such a characteristic make this SPI-based hydrogel an injectable hydrogel, which may have the considerable potential in the biomedicine field. However, the mechanical properties of current PEG modified SPI hydrogel is still not high enough(storage modulus of 300 Pa), so further effort should be taken to make it meet the requirement of real applications.

Key words: Soy protein isolate, Polyethylene glycol monomethyl ether, Chemical modification, Hydrogel

CLC Number:

O636

TrendMD:

LIU Jie, ZHOU Hao, HUANG Yufang, CHEN Xin. Polyethylene Glycol Chemically Modified Soy Protein Isolate Hydrogel^†[J]. Chem. J. Chinese Universities, 2018, 39(2): 390.

Figures/Tables 7

Scheme 1 Synthesis of mPEG-CHO

Fig.1 FTIR(A) and 1H NMR(B) spectra of mPEG-OH(a) and mPEG-CHO(b)

Scheme 2 Synthesis of mPEG modified SPI

Fig.2 FTIR(A) and 1H NMR spectra(B) of pristine SPI(a), SPI-mPEG10∶1(b) and SPI-mPEG20∶1(c)

Fig.3 TEM images of pristine SPI(A) and mPEG modified SPI(B)

Fig.4 Optical images of pristine SPI(A, B) and mPEG modified SPI(C, D) solution before(A, C) and after(B, D) incubation at 37 ℃ Solution concentration: 10%.

Fig.5 Rheological test of mPEG modified SPI hydrogel at 37 ℃ (A) Strain sweep; (B) angular frequency sweep.

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