Detection of Cefoperazone Sodium and Sulbactam Sodium in Biological Fluid by Quantum Dots Room Temperature Phosphorescence Probe†

doi:10.7503/cjcu20160082

Abstract

Abstract:

A method for the determination of trace cefoperazone sodium-sulbactam sodium(CPZ-SBT) was established using water phase synthesized 3-mercaptopropionic acid-capped Mn-doped ZnS quantum dots(MPA-Mn/ZnS QDs) as a room temperature phosphorescence(RTP) probe, which based on the phenomenon that the electron acceptor acted by CPZ-SBT can effectively quench the RTP of MPA-Mn/ZnS QDs through electron transfer. There is a good relationship between CPZ-SBT and changes on the phosphorescence intensity of MPA-Mn/ZnS QDs when the concentration of CPZ-SBT is in the scope of 0.7—84 μg/L. The correlation coefficient and detection limit of this method are 0.99 and 0.14 μg/L, respectively. Additionally, this method is able to conduct analysis simply without deoxidizer, inducer or complex sample processing, which can effectively avoid the interference of background fluorescence and scattered light in biological fluids and can be successfully applied in trace determination of CPZ-SBT in biological fluids as well.

Key words: Quantum dots, Room temperature phosphorescence probe, Detection, Cefoperazone sodium-sulbactam sodium(CPZ-SBT)

CLC Number:

O657.3

TrendMD:

FANG Xiaoxing,ZHENG Ji,YAN Guiqin. Detection of Cefoperazone Sodium and Sulbactam Sodium in Biological Fluid by Quantum Dots Room Temperature Phosphorescence Probe^†[J]. Chem. J. Chinese Universities, 2016, 37(8): 1435.

Figures/Tables 10

Fig.1 TEM image(A), XRD pattern(B), zeta potential diagram(C) and the excitation spectrum(a) with phosphorescence emission spectrum(b) of MPA-Mn/ZnS QDs(D) QDs: 40 mg/L; PBS buffer: 20 mmol/L; pH=6.0.

Fig.2 Effects of pH(A), time(B) and NaCl concentration(C) on the RTP intensity of QDs and QDs-CPZ-SBT

Fig.3 CPZ-SBT concentration-dependent RTP emission of the QDs(A) and the variation of ΔP(P0-P) as a function of CPZ-SBT QDs: 40 mg/L; CPZ-SBT: 84 μg/L; PBS: 20 mmol/L, pH=6.0.

Table 1 Different analytical techniques reported for detection of CPZ-SBT

Substance	Method	Detection range/(g·L^-1)	LOD/(μg·L^-1)	Reference
CPZ	HPLC	1.5×10^-2—2.7	0.408	[17]
SBT	HPLC	1.4×10^-2—2.7	1.96	[17]
CPZ	RP-HPLC	4.9×10^-2—0.49	1.0	[18]
SBT	RP-HPLC	2.4×10^-2—0.24	2.0	[18]
CPZ	UPLC	20.66—154.95	2.31	[19]
SBT	UPLC	20.96—152.18	6.22	[19]
CPZ-SBT	Fluorescence	8.0×10^-6—1.0×10^-4	0.10	[20]
CPZ-SBT	Phosphorescent	7.0×10^-7—8.4×10^-5	0.14	This method

Fig.4 Temperature-dependent response of P0/P to CPZ-SBT(A) and UV-Vis spectra of CPZ-SBT with emission spectra of QDs(B) QDs: 40 mg/L; CPZ-SBT: 84 μg/L. (B) a. CPZ-SBT; b. QDs.

Fig.5 Fluorescence(A), UV-Vis absorption(B), RLS of QDs-CPZ-SBT(C) spectra and changes of QDs-CPZ-SBT RLS after addition of NaCl(D)(QDs, 40 mg/L; CPZ-SBT, 84 μg/L) (A) a. CPZ-SBT; b. QDs; c. QDs-CPZ-SBT(QDs: 40 mg/L; CPZ-SBT: 84 μg/L); (B) a. CPZ-SBT; b. QDs; c. QDs-CPZ-SBT(QDs: 40 mg/L; CPZ-SBT: 42 μg/L); d. QDs-CPZ-SBT(QDs: 40 mg/L; CPZ-SBT: 84 μg/L); (C) QDs: 40 mg/L; concentration of CPZ-SBT/(μg·L-1): 0, 14, 28, 42, 56, 84; (D) QDs: 40 mg/L; CPZ-SBT: 84 μg/L; NaCl: 0.04, 0.10, 0.20, 0.30 mol/L.

Scheme 1 Mechanism of the RTP quenching process

Table 2 Tolerance of foreign substances

Co-existing substance	Tolerance ratio	Change of RTP intensity(%)	Co-existing substance	Tolerance ratio	Change of RTP intensity(%)
Na⁺	200	1.7	Serine	300	1.6
K⁺	200	-3.1	L-Gly	200	3.3
Ca²⁺	150	1.7	L-Cys	150	-1.9
Mg²⁺	100	-2.8	Glucose	100	2.6

Table 3 Detection of CPZ-SBT in real samples(n=3)

Sample	Added/(μg·L^-1)	Found/(μg·L^-1)	Recovery(%)	RSD(%)(n=3)
Human serum	5	4.6	95.3±2	2.78
	10	10.3	101.5±1	1.09
Human urine	5	5.2	103.7±4	1.96
	10	9.8	97.4±2	2.89

Fig.6 Phosphorescence and fluorescence spectra of urine(a, d) and serum(b, c)

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