Anti-diabetic Drugs Detection by Raman Spectrometry with Molecular Imprinted Composite Membrane†

doi:10.7503/cjcu20150533

Abstract

Abstract:

Some artificially synthesized anti-diabetic drugs are frequently found in health products to enhance their curative efficacy. Based on the specific recognition characteristics of molecularly imprinted technology, two kinds of molecularly imprinted composite membranes were prepared with dummy template of guanidine hydrochloride and 4-(2-aminoethyl)-benzene sulfonamide. The adsorption capacities of these two membranes to metformin hydrochloride, phenformin hydrochloride and glibenclamide were studied. Meanwhile, surface enhancement Raman spectrometry(SERS) to determine the target chemicals was established and employed in the membrane detection after infiltrated in the health products extract solution. The influence of nano-silver particles on the membrane to SERS was also studied. The detection limits of metformin hydrochloride, phenformin hydrochloride and glibenclamide are 5, 5 and 10 mg/mL, respectively. This method was also proved by several pieces of real samples.

Key words: Health care product, Anti-diabetic drug, Molecularly imprinted membrane, Nano-silver, Raman spectroscopy

CLC Number:

O657.37

TrendMD:

JIA Hua, YIN Ruilin, ZHONG Xu, XUE Min, ZHAO Yu, MENG Zihui, WANG Qunjie. Anti-diabetic Drugs Detection by Raman Spectrometry with Molecular Imprinted Composite Membrane^†[J]. Chem. J. Chinese Universities, 2016, 37(2): 239.

Figures/Tables 11

Fig.1 Chemical structures of dummy templates and anti-diabetic drugs

Fig.2 UV-Vis spectrum of nano-silver particles

Fig.3 SEM image of nano-silver particles

Fig.4 SEM images of membrane matrix(A), MIM(B) and MIM-Ag(C)

Fig.5 Raman spectra of metformin hydrochloride(A), MIM(a, c) and MIM-Ag(b, d) before(a, b) and after(c, d) adsorption of 75 mg/mL metformin hydrochloride(B)

Fig.6 Raman spectrum of phenformin hydrochloride

Fig.7 Raman spectra of MIM-Ag before and after adsorption of different concentrations of metformin hydrochloride(A) and phenformin hydrochloride(B) a. MIM-Ag(before adsorption); concentration/(mg·mL-1), b—g: 5, 9, 19, 38, 75, 100.

Fig.8 Raman spectra of MIM-Ag before(a) and after(b) adsorption of a real sample (No.JC1012998) extract

Fig.9 Raman spectra of MIM-Ag after adsorption of a real sample(No.JC1012998) extract(a) and rinsed with chloroform(b)

Fig.10 Raman spectra of glibenclamide(A) and B-MIM-Ag before(a) and after adsorption(b—d)(B) (B) b—d. B-MIM-Ag adsorbs 5, 12 and 15 mg/mL gibenciamide, respectively.

Fig.11 Raman spectra of B-MIM-Ag before(a) and after adsorption of a real sample (No.JC1300635) extract(b) and after adsorption of the sample(No.JC1300635) extract added standard(c)

References 26

[1]	Yang Z., Chen A. Z., Wu A. Y., Li X. R., Chin. J. Pharm. Anal., 2009, 29(12), 2127—2130
	(杨钊, 陈安珍, 吴爱英, 李欣荣. 药物分析杂志, 2009, 29(12), 2127—2130)
[2]	Zhang S., Xue L., Zhang T., Rao W. W., Drug Stand. China, 2003, 4(6), 37—39
	(张穗, 薛漓, 张涛, 饶伟文. 中国药品标准, 2003, 4(6), 37—39)
[3]	Zhou Z. G., Zhang J. L., Zhang W., Analyst, 2011, 136(12), 2613—2618
[4]	Zhang Y. L., Cao L., Pharm. Clin. Res., 2007, 15(5), 423—426
	(张妤琳, 曹玲. 药学与临床研究, 2007, 15(5), 423—426)
[5]	Cao H. B., Yuan Y., Herald Med., 2011, 29(10), 1358—1360
	(曹恒斌, 袁玉梅. 医药导报, 2011, 29(10), 1358—1360)
[6]	Chen H. L., Zhang X. B., Strait Pharm. J., 2011, 23(11), 70—72
	(陈海雷, 张学斌. 海峡药学, 2011, 23(11), 70—72)
[7]	Jiang R. G., Li J. W., Zhang T. H., Zhao C. S., He Z. G., J. Shenyang Pham. Univ., 2003, 20(4), 272—274
	(江荣高, 李建文, 张天虹, 赵春顺, 何仲贵. 沈阳药科大学学报, 2003, 20(4), 272—274)
[8]	Havele S. S., Dhaneshwar S. R., Chem. Ind. Chem. Eng. Q., 2014, 20(1), 39—47
[9]	Zeng M. F., Zhang X. B., Chin. J. Mod. Appl. Pharm., 2006, 23(6), 506—507
	(曾茂法, 张学斌. 中国现代应用药学杂志, 2006, 23(6), 506—507)
[10]	Feng S. Y., Lai E. P. C., Dabek-Zlotorzynska E., Sadeghi S., J. Chromatogr. A, 2004, 1027(1/2), 155—160
[11]	Lahsini R., Louhaichi M. R., Adhoum N., Monser L., Acta Pharm., 2013, 63(2), 265—275
[12]	Liu Z. B., Jia F. Y., Wang W. W., Wang C. X., Liu Y. M., Lumin., 2012, 27(4), 297—301
[13]	Hu J., Liang Y. R., Xia D. H., Ye Y. H., Wu P. X., Cent. South Pharm., 2006, 4(4), 296—297
	(胡剑, 梁仪容, 夏德豪, 叶玉华, 伍鹏兮. 中南药学, 2006, 4(4), 296—297)
[14]	Zhang Y., Huang X. Y., Liu W. F., Cheng Z. N., Chen C. P., Yin L. H., Anal. Sci., 2013, 29, 985—990
[15]	Hu Y. X., Feng S. L., Gao F., Food Chem., 2015, 176, 123—129
[16]	Gao F., Feng S. L., Chen Z. W., J. Food Sci., 2014, 79(12), 2542—2549
[17]	Gultekin A., Diltemiz S. E., Ersoz A., Sariozlu N. Y., Denizli A., Say R., Talanta, 2009, 78(4/5), 1332—1338
[18]	Zhang W. Y., Xiao X. Z., Liu X. Q., Zhang R., Xu Y., Chem. J. Chinese Universities, 2013, 34(6), 1385—1388
	(张文译, 肖鑫泽, 刘学青, 张然, 徐颖. 高等学校化学学报, 2013, 34(6), 1385—1388)
[19]	Zhang J., Wang C. Y., Li X. P., Niu Y. H., Chem. J. Chinese Universities, 2013, 34(10), 2296—2302
	(张进, 王超英, 李小平, 牛延慧. 高等学校化学学报, 2013, 34(10), 2296—2302)
[20]	Lee P. C., Meisel D., J. Phys. Chem., 1982, 86(17), 3391—3395
[21]	Yin J.F., Meng Z. H., Du M. J., Song M. Y., Wang H. L.,National Symposium on Biomedical Chromatography, 2010, 251—252
	(尹俊发, 孟子晖, 都明君, 宋茂勇, 汪海林. 全国生物医药色谱学术交流会, 2010, 251—252)
[22]	Chen L. X., Xu S. F., Li J. H., Chem. Soc. Rev., 2011, 40(5), 2922—2942
[23]	Xu S. F., Lu H. Z., Li J. H., ACS Appl. Mater. Interfaces, 2013, 5(16), 8146—8154
[24]	Zhu X. F., Cao X. F., Cao Q. E., Wang G. S., Hou N. B., Ding Z. T., Chin. J. Anal. Chem., 2006, 34, 118—122
	(朱秀芳, 曹秋娥, 汪国松, 侯能邦, 丁中涛. 分析化学, 2006, 34, 118—122)
[25]	Liu G.X., Zhu L. H., Shen X. T., Tang H. Q.,The 4th National Academic Conference of Enviromental Chemistry, 2007, 387—388
	(刘国霞, 朱丽华, 沈先涛, 唐和清. 第四届全国环境化学学术大会, 2007, 387—388)
[26]	Yin L. H., Zhang Y., Chin. J. Pharm. Anal., 2010, 30(12), 2352—2355
	(尹利辉, 张雁. 药物分析杂志, 2010, 30(12), 2352—2355)