Liquid Crystal Biosensor Based on Growth of Gold Nanoparticles for Detection of Tyrosine

doi:10.7503/cjcu20130536

Abstract

Abstract:

A new-style liquid crystal(LC) biosensor based on enzymatic growth of gold nanoparticles(AuNPs) was developed for detection of tyrosine(Tyr). Tyrosinase(TR) was immobilized on the glass slides modified with APTES/DMOAP mixed self-assembled monolayers(SAM) and activated by glutaraldehyde, which, afterwards, were immersed in a growth solution containing different concentrations of Tyr. In this me-thod, TR stimulated the biocatalyzed hydroxylation of Tyr to L-Dopa which could reduce Au³⁺ in the growth solution into AuNPs. The formation of AuNPs could greatly change the surface topology and induce a homeotropic-to-tiled transition of the LC molecules, resulting in significant change in corresponding of optical appea-rances under the crossed polarized light. The results showed that the presented LC biosensor can highly sensitive to Tyr with a limit of detection of 6×10^-7 mol/L. Furthermore, this study offers a simple, high-specific and label-free method with naked eye for Tyr.

Key words: Liquid crystal, Self-assembly, Tyrosine, Tyrosinase, Gold nanoparticles(AuNPs)

CLC Number:

O657.39

TrendMD:

LI Guang, CHEN Long-Cong, CHEN Meng-Meng, GAO Bin, XIONG Xing-Liang. Liquid Crystal Biosensor Based on Growth of Gold Nanoparticles for Detection of Tyrosine[J]. Chem. J. Chinese Universities, 2013, 34(11): 2493.

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