Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (6): 1360.doi: 10.7503/cjcu20120913

• Analytical Chemistry • Previous Articles     Next Articles

Interaction of Herring Sperm DNA with Lomefloxacin Hydrochloride-Cu(Ⅱ) Based on Changes in the Fluorescence Intensity of GSH-CdTe/CdS Quantum Dots

SHEN Yi-Zhong, LIU Shao-Pu, YIN Peng-Fei, HE You-Qiu   

  1. School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
  • Received:2012-10-09 Online:2013-06-10 Published:2013-01-07

Abstract:

Glutathione(GSH)-capped CdTe/CdS quantum dots(GSH-CdTe/CdS QDs) were synthesized in aqueous solution. The particle sizes and morphological characteristics of GSH-CdTe/CdS QDs were investigated by transmission electron microscopy(TEM). The results exhibits that the particle size of as-prepared QDs has a narrow size distribution and good dispersivity. In Tris-HCl buffer medium(pH=7.6), lomefloxacin hydrochloride-Cu(Ⅱ) coordination compound(LMFH-Cu2+) was adsorbed to the surfaces of GSH-CdTe/CdS QDs through electrostatic attraction and formed ground state complex, which resulted in the quenching of the fluorescence of GSH-CdTe/CdS QDs. Adding herring sperm DNA(hsDNA) to GSH-CdTe/CdS QDs-LMFH-Cu(Ⅱ) system led to the fluorescence intensity of GSH-CdTe/CdS QDs recover, which can be explained by that the addition of hsDNA to the system induced LMFH-Cu(Ⅱ) to dissociate from the surface of GSH-CdTe/CdS QDs and embed into its double helix structure. According to the fluorescence quenching and restoration for GSH-CdTe/CdS QDs, fluorescence reversible control of GSH-CdTe/CdS QDs was realized. Compared with the interaction between GSH-CdTe/CdS QDs and LMFH, the interaction of GSH-CdTe/CdS QDs-LMFH-Cu(Ⅱ)-hsDNA was studied by fluorescence(FL), resonance Rayleigh scattering(RRS) and ultraviolet-visible absorption(UV-Vis) spectra. Meanwhile, the interaction mechanism was discussed and corresponding model of interaction was built.

Key words: GSH-CdTe/CdS quantum dot(QD), Lomefloxacin hydrochloride-Cu(Ⅱ), Herring sperm DNA, Fluorescence reversible control

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