A Tetrapeptide Containing Selenium(SecRGD) with Glutathione Peroxidase Activity

doi:10.3969/j.issn.0251-0790.2012.05.021

Abstract

Abstract: Glutathione peroxidase(GPx) is the most crucial antioxidant enzyme in a variety of organisms. Here, base on the Arg-Gly-Asp(RGD), a tetrapeptide containing selenium(SeCRGD) was chemically synthesized. The existential state of the peptide was investigated by ESI-MS and hydride generation atom fluorescence spectral analysis. The GPx activities and the kinetics of the peptide were determined by the enzyme coupling method. The antioxidant effect of the peptide was evaluated based on MTT assay. The results showed that a kind of oxide form, SecRGD-dimer, was confirmed by ESI-MS and selenium content analysis. The peptide had GPx activity of 5.54 U/μmol and more than that of Ebselen. As native enzyme, a ping-pong mechanism was observed in steady-state kinetics studies. Moreover, the novel selenopeptide occupied merits of small molecular weight, good solubility in water and low toxicity. Especially, the peptide was found that could protect Vero cells from the injury induced by H₂O₂. Thus, the novel selenopeptide possesses a good potential in medicinal application.

Key words: Glutathione peroxidase, Selenopeptide, Antioxidant activity

CLC Number:

O627.6

TrendMD:

ZOU Xian-Feng, LV Shao-Wu. A Tetrapeptide Containing Selenium(SecRGD) with Glutathione Peroxidase Activity[J]. Chem. J. Chinese Universities, 2012, 33(05): 983.

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A Tetrapeptide Containing Selenium(SecRGD) with Glutathione Peroxidase Activity

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