Specific Assay of Amino Acids Based on a Fluorescent Conjugated Polyelectrolyte with Multi-carboxylates

doi:10.3969/j.issn.0251-0790.2012.01.009

Abstract

Abstract: A simple assay for cysteine(Cys) and histidine(His) based on a conjugated polyelectrolyte with multi-carboxylate groups was developed. The proposed approach takes advantage of the emission quenching of PPE-(COOK)₄ caused by Cu²⁺, which can be efficiently restored in the presence of Cys or His. Thus, the rapid assay of both amino acids can be carried out by measuring the fluorescence recovery. With the high similarity between the polymer’s side groups and EDTA, PPE-(COOK)₄ in phosphate buffer solution exhibits not only high sensitivity but remarkable specificity towards Cu²⁺, which makes it possible to introduce various shielding metals for improving the selectivity of Cys and His assays. The results demonstrated that both Cys and His can be assayed sensitively and selectively by adding Ni²⁺ or Hg²⁺ to PPE-(COOK)₄/Cu²⁺ system. The limit of detection is 0.15 μmol/L for Cys and 0.04 μmol/L for His, with the linear range of 1-5 μmol/L and 0.5-2.5 μmol/L, respectively.

Key words: Conjugated polyelectrolyte, Biosensor, Cu²⁺ probe, Amino acid

CLC Number:

O656

TrendMD:

FAN Hong-Liang, ZHANG Tao, LI Zu-Hong, WU Li-Yuan, JIN Qin-Han. Specific Assay of Amino Acids Based on a Fluorescent Conjugated Polyelectrolyte with Multi-carboxylates[J]. Chem. J. Chinese Universities, 2012, 33(01): 54.

References

[1] Zhou Q., Swager T. M.. J. Am. Chem. Soc.[J], 1995, 117: 12593-12602 [2] Chen L. H., McBranch D. W., Wang H. L., Helgeson R., Wudl F., Whitten D. G.. Proc. Natl. Acad. Sci. USA[J], 1999, 96: 12287-12292 [3] DiCesare N., Pinto M. R., Schanze K. S., Lakowicz J. R.. Langmuir[J], 2002, 18: 7785-7787 [4] Zhang T., Fan H. L., Liu G. L., Jiang J., Zhou J. G., Jin Q. H.. Chem. Commun.[J], 2008, (42): 5414-5416 [5] Miranda O. R., You C. C., Phillips R., Kim I. B., Ghosh P. S., Bunz U. H. F., Rotello V. M.. J. Am. Chem. Soc.[J], 2007, 129: 9856-9857 [6] Shang L., Qin C. J., Wang T., Wang M., Wang L. X., Dong S. J.. J. Phys. Chem. C[J], 2007, 111: 13414-13417 [7] Zhang Y., Wang Y., Liu B.. Anal. Chem.[J], 2009, 81: 3731-3737 [8] Jiang H., Zhao X. Y., Schanze K. S.. Langmuir[J], 2006, 22: 5541-5543 [9] YANG Xiao-Hai(羊小海), WANG Sheng-Feng(王胜锋), WANG Ke-Min(王柯敏), LUO Xiao-Ming(罗晓明), TAN Wei-Hong(谭蔚泓), CUI Liang(崔亮). Chem. J. Chinese Universities(高等学校化学学报)[J], 2009, 30(5): 899-902 [10] Feng F., Tang Y., Wang S., Li Y., Zhu D.. Angew. Chem. Int. Ed.[J], 2007, 46: 7882-7886 [11] Liu Y., Schanze K. S.. Anal. Chem.[J], 2008, 80: 8605-8612 [12] Gaylord B. S., Heeger A. J., Bazan G. C.. Proc. Natl. Acad. Sci. USA[J], 2002, 99: 10954-10957 [13] Ho H. A., Boissinot M., Bergeron M. G., Corbeil G., Dore K., Boudreau D., Leclerc M.. Angew. Chem. Int. Ed.[J], 2002, 41: 1548-1551 [14] Zhang T., Fan H. L., Zhou J. G., Jin Q. H.. J. Polym. Sci., Part A: Polym. Chem.[J], 2009, 47: 3056-3065 [15] Fan H. L., Zhang T., Lv S. W., Jin Q. H.. J. Mater. Chem.[J], 2010, 20: 10901-10907 [16] Lakowicz J. R.. Principles of Fluorescence Spectroscopy[M], New York: Plenum Press, 1983: 277-284 [17] Zhang T., Fan H. L., Zhou J. G., Liu G. L., Feng G. D., Jin Q. H.. Macromolecules[J], 2006, 39: 7839-7843 [18] ZHANG Tao(张涛), FAN Hong-Liang(范宏亮), ZHOU Jian-Guang(周建光), LIU Guo-Liang(刘国良), JIN Qin-Han(金钦汉). Chem. J. Chinese Universities(高等学校化学学报)[J], 2006, 27(5): 853-855