高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (6): 1026.

• 研究论文 • 上一篇    下一篇

用化学振荡(乳酸-丙酮-BrO3--Mn2+-H2SO4体系)“探针”检测谷胱甘肽的抗氧化效应

杨树涛, 甘南琴, 林智信, 蔡汝秀   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:2001-07-27 出版日期:2002-06-24 发布日期:2002-06-24
  • 通讯作者: 蔡汝秀(1939年出生),女,教授,博士生导师,主要从事动态分子光谱和分析化学动力学研究.E-mail:dlxfxc@w hu.edu.cn E-mail:dlxfxc@w hu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20075018)资助

Determination of Activity of Antioxygenation of Glutathione by the "Probe" of the Lactic Acid(LA)-Acetone(Act)-BrO3--Mn2+-H2SO4 Oscillating System

YANG Shu-Tao, GAN Nan-Qin, LIN Zhi-Xin, CAI Ru-Xiu   

  1. Department of Chemistry, Wuhan University, Wuhan 430072, China
  • Received:2001-07-27 Online:2002-06-24 Published:2002-06-24

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摘要： 利用分析物脉冲微扰(Analyte Pulse Perturbation,APP)技术研究了生物体内的一种抗氧化剂谷胱甘肽对乳酸-丙酮-BrO3--Mn2+-H2SO4化学振荡体系振荡规律的影响.采用这一振荡体系作为探针来检测GSH的抗氧化效应,考察了振荡体系中各反应物的浓度及各种因素的影响,它可作为定量分析的基础,线性范围为1.50×10-7～2.25×10-5mol/L,检测限达2.20×10-9mol/L,RSD为1.35%(n=11),测定样品的速率为每小时6～9个样品,分析了可能的反应机理.

关键词: 谷胱甘肽, 乳酸, APP技术, 抗氧化效应

Abstract: In our experiment, we applied APPtechnique to study of the perturbation of glutathione(GSH) which is an important antioxidant in vivo on the lactic acid(LA) Acetone(Act) BrO3--Mn2+-H2SO4 oscillating reaction. And we determinate the activity of antioxygenation of GSHby the "probe" of this oscillating system. Influence of experiment variables were investigated, and can be as a base of quantitative analysis. The linear range lies between 1.50×10-7-2.25×10-5mol/L, and the determination limit is 2.20×10-9mol/L. And the precision and throughput were also quite good(1.35% as RSD, and 6-9 samples/h, respctively). Some aspects of the potential mechanism of action GSHon the oscillating systems are discussed.

Key words: Glutathione, Lactic acid, APP technique, Antioxygenatic activity

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