高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (9): 1577.

• 论文 • 上一篇    下一篇

刺五加寡糖的电喷雾多级串联质谱研究

郭明全, 宋凤瑞, 陈貌连, 白玉, 刘志强, 刘淑莹   

  1. 中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:2002-03-29 出版日期:2003-09-24 发布日期:2003-09-24
  • 通讯作者: 刘淑莹(1942年出生),女,博士,研究员.博士生导师,从事质谱研究.E-mail:mslab@ns.ciac.jl.cn;刘志强(1962年出生),男,博士,研究员,博士生导师,从事质谱研究. E-mail:mslab@ns.ciac.jl.cn
  • 基金资助:

    吉林省科技厅重点发展项目(批准号:19990310-2)资助

Studies on Oligosaccharides from Acanthopanax Senticosus Harms (AS) by Electrospray Ionization Tandem Mass Spectrometry(MSn)

GUO Ming-Quan, SONG Feng-Rui, CHEN Mao-Lian, BAI Yu, LIU Zhi-Qiang, LIU Shu-Ying   

  1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2002-03-29 Online:2003-09-24 Published:2003-09-24

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被引次数

48

摘要： 采用小柱层析法从刺五加中分离得到刺五加寡糖类系列化合物(刺五加二糖刺五加六糖).实验结果表明,在正离子模式下的ESI-MS谱中,此类化合物呈现出特征的加合离子峰簇[M+Na]+/[M+K]+或[M+H2O+Na]+/[M+H2O+K]+,可以确定其分子量;在负离子模式下的ESI-MS谱中,刺五加寡糖易形成[M-H]-/[M+nH2O-H]-(n<3).还利用电喷雾多级串联质谱(ESI-MSn)对刺五加三糖进行了系统的研究,推断出刺五加三糖的组成与结构.

关键词: 刺五加, 寡糖, 电喷雾多级串联质谱

Abstract: Aseries of oligosaccharide mixtures from extracts of Acanthopanax senticosus Harms(AS) were determined in both the positive ion mode and negative ion mode of electrospray ionization tandem mass spectrometry. In the positive ion mode, the ions observed are characteristic of cluster ions with a mass difference of 16, and they are considered as the alkali adducts of [M+Na]+/[M+K]+or [M+H2O+Na]+/[M+H2O+K]+. In the negative ion mode, the ions observed are deprotonated oligosaccharides like [M-H]-or [M+H2O-H]-. MS" experiments of trisaccharides were performed in both the positive and negative ion modes to highlight the application of ESI-MSnto the structure analyses of oligosaccharides.

Key words: Acanthopanax senticosus, Oligosaccharide, Electrospray ionization tandem mass spectrometry

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