高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (5): 1181.

• 研究论文 • 上一篇    下一篇

电活性可生物降解纳米复合材料PAP/op-HA/PLGA的制备及成骨活性

邬海涛1,2, 于婷3, 朱庆三2, 焦自学1,  危岩4,  章培标1, 陈学思1   

  1. 1. 中国科学院长春应用化学研究所, 高分子物理与化学国家重点实验室, 长春  130022;
    2. 吉林大学第一医院, 长春 130021;
    3. 吉林大学第二医院, 长春 130041;
    4. 清华大学化学系, 北京 100084
  • 收稿日期:2010-12-07 修回日期:2011-03-02 出版日期:2011-05-10 发布日期:2011-04-11
  • 通讯作者: 章培标;朱庆三 E-mail:zhangpb@ciac.jl.cn;drzqs@yahoo.com.cn
  • 基金资助:

    国家“八六三”计划项目(批准号： 2007AA03Z320)和国家自然科学基金面上项目(批准号:   50673090)资助.

Preparation and Osteogenesis Activities of  Electroactive Biodegradable Nanocomposites PAP/op-HA/PLGA

WU Hai-Tao1,2, YU Ting3, ZHU Qing-San2*, JIAO Zi-Xue1, WEI Yan4, ZHANG Pei-Biao1*, CHEN Xue-Si1   

  1. 1.  Key Laboratory of Polymer Ecobiomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; 
    2.  First Hospital, 
    3.  Second Hospital, Jilin University, Changchun 130041, China;
    4.  Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2010-12-07 Revised:2011-03-02 Online:2011-05-10 Published:2011-04-11
  • Contact: ZHANG Pei-Biao;ZHU Qing-San E-mail:zhangpb@ciac.jl.cn;drzqs@yahoo.com.cn
  • Supported by:

    国家“八六三”计划项目(批准号： 2007AA03Z320)和国家自然科学基金面上项目(批准号:   50673090)资助.

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被引次数

12

摘要： 以苯胺五聚体与聚乳酸（PLA）的三嵌段共聚物（PAP）与表面接枝低聚乳酸的纳米羟基磷灰石（op-HA）和聚丙交酯-乙交酯（PLGA）的复合物共混，制备的电活性可降解纳米复合材料PAP/op-HA/PLGA为研究对象，采用紫外可见光谱、循环伏安扫描和标准四探针法分析其电化学特性及电导率。ESEM观察其膜表面形貌，接触角评价其亲水性。通过在材料膜表面接种兔成骨细胞进行体外培养，采用荧光染色、NIH Image J图像分析和Real-time PCR综合评价细胞在材料表面的粘附、扩展（细胞面积比）和成骨相关基因的表达水平，以此评价新型电活性纳米复合骨修复材料PAP/op-HA/PLGA的表面性质和生物活性。结果表明，PAP/op-HA/PLGA的电导率较低（~5?10?6 S/cm）但具有良好的电化学氧化还原性能，PAP含量为0.1%时材料的亲水性明显改善，成骨细胞的粘附和扩展明显增强。培养7天时骨形态蛋白- 2（BMP-2）和骨连接蛋白（Osteonectin）的基因表达水平明显提高，而对Ⅰ型胶原蛋白的基因表达没有明显影响。提示PAP/op-HA/PLGA具有良好的细胞相容性和成骨活性。

关键词: 苯胺五聚体, 嵌段共聚物, 纳米羟基磷灰石, 聚丙交酯-乙交酯（PLGA）, 导电高分子, 成骨活性

Abstract: We prepared the electroactive biodegradable nanocomposites PAP/ op-HA/PLGA by blending a triblock copolymer PLA-b-AP-b-PLA (PAP) of polylactide (PLA) and aniline pentamer (AP) with oligomeric lactic acid-surface grafted hydroxyapatite (op-HA)/poly(lactide-co-glycolide)(PLGA). The electrochemical properties and conductivity were characterized using UV-Vis spectroscopy, cyclic voltammetry and standard four-probe method. Film’s surface morphology was displayed by ESEM and contact angle was measured to evaluate the film’s hydrophilicity. Rabbit’s osteoblasts were cultured in vitro on the film surface. For comprehensively evaluating surface properties and biological activity of the new electroactive nanocomposites PAP/op-HA/PLGA for bone repair, cell adhesion and cell spreading (cell area fraction) were determined using fluorescein isothiocyanate dyeing and NIH Image J software analysis, and gene expression levels were detected using Real-time PCR. The results show that PAP/op-HA/PLGA had good electrochemical redox property and low conductivity (~ 5 ? 10-6 S/cm). With the content of 0.1 wt% PAP, the hydrophilicity of composites was improved, the adhesion and expansion of osteoblasts were enhanced, and bone morphogenetic protein-2 (BMP-2) and bone junction protein (osteonectin) gene expression levels were increased, significantly. In conclusion, PAP/op-HA/PLGA composites have good biocompatibility and osteogenic activity.

Key words: Aniline pentamer, Block copolymers, Nano-hydroxyapatite, poly(lactide-co-glycolide), Conductive polymer, Osteogenic activity

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