聚乳酸-乙醇酸/纳米氧化锌复合电纺纤维装载亲疏水药物的控释及体外细胞毒性

doi:10.7503/cjcu20140359

高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (8): 1788.doi: 10.7503/cjcu20140359

聚乳酸-乙醇酸/纳米氧化锌复合电纺纤维装载亲疏水药物的控释及体外细胞毒性

曾莉^1,², 胡俊², 魏俊超²()

1. 江西省化学工业学校, 南昌 330012
2. 南昌大学化学系, 南昌 330031

收稿日期:2014-04-16 出版日期:2014-08-10 发布日期:2019-08-01
作者简介:联系人简介:魏俊超, 男, 博士, 副教授, 主要从事生物医用高分子材料、纳米复合材料研究. E-mail: weijunchao@ncu.edu.cn
基金资助:
基金项目:国家自然科学基金(批准号: 51203073)和江西省教育厅科学基金(批准号: GJJ13107)资助.

Controlled Release of Multiple Hydrophilic and Hydrophobic Drugs and in vitro Cytotoxicity of Electrospun Poly(lactic-co-glycolic acid)/ZnO Nanofibers Encapsulated with Dual Drugs^†

ZENG Li^1,², Hu Jun¹, WEI Junchao^2,^*()

1. School of Chemical Industry of Jiangxi Province, Nanchang 330012, China
2. Department of Chemistry, Nanchang University, Nanchang 330031, China

Received:2014-04-16 Online:2014-08-10 Published:2019-08-01
Contact: WEI Junchao E-mail:weijunchao@ncu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.51203073) and the Natural Science Foundation of Education Department of Jiangxi Province, China(No.GJJ13107).

摘要/Abstract

摘要：

利用静电纺丝技术制备了负载亲水性药物阿霉素(DOX)以及疏水性药物喜树碱(CPT)的复合纳米纤维. 先用巯基封端的普朗尼克(F127)修饰纳米氧化锌(FZnO), 再将FZnO负载盐酸阿霉素(DOX@FZnO), 最后将DOX@FZnO与CPT一起纺入聚乳酸-乙醇酸(PLGA)纤维中. 体外药物释放结果表明, 复合纳米纤维能够减小亲水性药物的突释, 减缓药物释放速率, 延长药物释放时间. 体外细胞活性结果表明, 双载药复合纤维比单载药复合纤维具有更强的细胞毒性, 能够有效抑制癌细胞生长.

关键词: 静电纺丝, 复合纳米纤维, 氧化锌, 药物控释, 细胞毒性, 聚乳酸-乙醇酸

Abstract:

A novel poly(lactic-co-glycolic acid)(PLGA)/ZnO electrospun composite fibers encapsulated with both hydrophilic drug(doxorubicin hydrochloride, DOX) and hydrophobic drug(camptothecin, CPT) were fabricated via electrospinning method. Primarily, the ZnO was decorat with F127 and then used to encapsulate DOX. Finally, the DOX-loaded ZnO(DOX@ZnO) and CPT were mixed with PLGA solution to fabricate electrospun hybrid nanofibers. The in vitro release results demonstrated the composite fibers decreased the burst release and increased the time of release, which showed a long-term and sustained release. The cell cytotoxicity test demonstated that the composite nanofiber with two drugs showed stronger antitumor efficacy against HepG-2 cells than the nanofiber with single drug. Thus, the composite nanofibers with two anticancer drugs could be a versatile drug delivery system as local implantable scaffolds for potential postsurgical cancer treatment.

Key words: Electrospinning, Nanofibers, ZnO, Drug delivery, Cytotoxicity, Poly(lactic-co-glycolic acid)

中图分类号:

O631

TrendMD:

曾莉, 胡俊, 魏俊超. 聚乳酸-乙醇酸/纳米氧化锌复合电纺纤维装载亲疏水药物的控释及体外细胞毒性. 高等学校化学学报, 2014, 35(8): 1788.

ZENG Li, Hu Jun, WEI Junchao. Controlled Release of Multiple Hydrophilic and Hydrophobic Drugs and in vitro Cytotoxicity of Electrospun Poly(lactic-co-glycolic acid)/ZnO Nanofibers Encapsulated with Dual Drugs^†. Chem. J. Chinese Universities, 2014, 35(8): 1788.

图/表 12

Fig.1 Schematic illustration of the process of fabrication of dual drug-loaded electrospun composite fiber

Fig.2 Synthesis of thiolated Pluronic F127

Fig.3 1H NMR spectra of F127(a) and HS-F127-SH(b)

Fig.4 FTIR spectra of ZnO NPs(a), HS-F127-SH(b) and FZnO NPs(c)

Fig.5 TGA curves of ZnO NPs(a) and FZnO NPs(b)

Fig.6 SEM images of ZnO NPs(A), FZnO NPs(B) and TEM image of FZnO NPs(C)

Fig.7 Size distribution of FZnO NPs

Fig.8 SEM images of neat PLGA fibers(A), FZnO/PLGA fibers(B), DOX@FZnO/PLGA fibers(C) and CPT/DOX@FZnO/PLGA fibers(D)

Fig.9 TEM images of neat PLGA fibers(A) and DOX@FZnO/PLGA fibers(B)

Fig.10 Cumulative DOX release from DOX@FZnO, DOX@FZnO/PLGA fibers and CPT/DOX@FZnO/PLGA composite fibers(A) and cumulative CPT release from CPT/DOX@FZnO/PLGA composite nanofibers(B)

Fig.11 In vitro cytotoxicity of the nanofiber mats to human liver carcinoma HepG-2 cells

Fig.12 CLSM images of HepG-2 cells co-cultured with blank(A), FZnO/PLGA fibers(B), DOX@FZnO/PLGA fibers(C) and CPT/DOX@FZnO/PLGA fibers(D)

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聚乳酸-乙醇酸/纳米氧化锌复合电纺纤维装载亲疏水药物的控释及体外细胞毒性

Controlled Release of Multiple Hydrophilic and Hydrophobic Drugs and in vitro Cytotoxicity of Electrospun Poly(lactic-co-glycolic acid)/ZnO Nanofibers Encapsulated with Dual Drugs^†

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聚乳酸-乙醇酸/纳米氧化锌复合电纺纤维装载亲疏水药物的控释及体外细胞毒性

Controlled Release of Multiple Hydrophilic and Hydrophobic Drugs and in vitro Cytotoxicity of Electrospun Poly(lactic-co-glycolic acid)/ZnO Nanofibers Encapsulated with Dual Drugs†

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Controlled Release of Multiple Hydrophilic and Hydrophobic Drugs and in vitro Cytotoxicity of Electrospun Poly(lactic-co-glycolic acid)/ZnO Nanofibers Encapsulated with Dual Drugs^†