MicroRNA的超灵敏检测研究进展

doi:10.7503/cjcu20160587

摘要/Abstract

摘要：

综述了最新发展的MicroRNA(miRNA)分析方法, 包括比色、荧光、化学发光、表面增强拉曼、单分子检测和电化学分析, 并对miRNA检测的发展方向做了展望.

Abstract:

MicroRNA(miRNA) is a class of endogenous noncoding RNA molecule with the length of about 22 nucleotides. MicroRNA may regulate more than 50% of human protein-coding genes, and plays important roles in regulating cell differentiation and other biological processes. The abnormal expression of miRNA is closely related to the occurrence of many diseases including cancers. Therefore, miRNA may function as a potential biomarker for molecular diagnostics. The sensitive detection of miRNA is essential to the early clinical diagnosis and the development of anticancer drugs. Because of the short size, low abundance in total RNA samples and the homologous similarities in miRNA family, the ultrasensitive detection of miRNA remains a great challenge. Recently, many new methods have been developed to improve the detection sensitivity. This review focuses on the advance in miRNA detection methods including colorimetric assay, fluorescent assay, chemiluminescent assay, surface enhanced Raman scattering, single molecules detection and electrochemical detection. In addition, we give a new insight into future development of miRNA assay.

Key words: MicroRNA, Ultrasensitive detection, Amplification, Analytical method

中图分类号:

O655

TrendMD:

王子月, 刘萌, 张春阳. MicroRNA的超灵敏检测研究进展. 高等学校化学学报, 2017, 38(1): 1.

WANG Ziyue, LIU Meng, ZHANG Chunyang. Advance in Ultrasensitive Detection of MicroRNA^†. Chem. J. Chinese Universities, 2017, 38(1): 1.

图/表 7

Fig.1 Scheme for miRNA detection with hairpin probe-based circular exponential amplification assay[41] Copyright from the American Chemical Society.

Fig.2 Schematic illustration of miRNA assay based on bifunctional SDA-Mediated HRCA[50] Copyright from the American Chemical Society.

Fig.3 Schematic illustration of miRNA assay using target-triggered recycling signal amplification in combination with 2-aminopurine probe[52] Copyright from the American Chemical Society.

Fig.4 Schematic illustration of RCA-amplified CXCLAmp strategy and its application for miRNA assay[55] Copyright from the Royal Society of Chemistry.

Fig.5 Schematic illustration of EXPAR-based SERS for microRNA assay[57] Copyright from the Royal Society of Chemistry.

Fig.6 Schematic illustration of quantum dot(QD)-based miRNA nanosensor for miRNA point mutation assay[61] Copyright from the Royal Society of Chemistry.

Fig.7 Schematic representation of the ratiometric ECL biosensor for microRNAs based on cyclic enzyme amplification and resonance energy transfer[66] Copyright from the Royal Society of Chemistry.

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