胞苷保护的铜纳米簇新型荧光探针检测重铬酸根离子

doi:10.7503/cjcu20170160

高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (10): 1737.doi: 10.7503/cjcu20170160

胞苷保护的铜纳米簇新型荧光探针检测重铬酸根离子

陈天霞¹, 张正涛¹, 王勇¹(), 倪永年^1,²()

1. 南昌大学化学学院, 南昌 330031
2. 南昌大学食品科学与技术国家重点实验室, 南昌 330047

收稿日期:2017-03-20 出版日期:2017-10-10 发布日期:2017-09-20
作者简介:联系人简介: 王勇, 男, 博士, 副教授, 主要从事分析化学和化学计量学方面的研究. E-mail:wangyong@ncu.edu.cn;倪永年, 男, 教授, 主要从事分析化学、化学计量学和生命分析化学方面的研究. E-mail:ynni@ncu.edu.cn
基金资助:
国家自然科学基金(批准号: 21305061)、江西省自然科学基金(批准号: 20171BAB203018, 20151BAB203021)、江西省教育厅科学技术研究项目(批准号: GJJ160006, GJJ160204)、南昌大学食品科学与技术国家重点实验室基金(批准号: SKLF-ZZA201612)和南昌大学研究生创新基金(批准号: cx2016053)资助

Cytidine-protected Copper Nanoclusters as a Novel Fluorescent Probe for the Detection of Dichromate Anion^†

CHEN Tianxia¹, ZHANG Zhengtao¹, WANG Yong^1,^*(), NI Yongnian^1,^2,^*()

Received:2017-03-20 Online:2017-10-10 Published:2017-09-20
Contact: WANG Yong,NI Yongnian E-mail:wangyong@ncu.edu.cn;ynni@ncu.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(No.21305061), the Natural Science Foundation of Jiangxi Province, China(Nos.20171BAB203018, 20151BAB203021), the Program of Jiangxi Provincial Department of Education, China(Nos.GJJ160006, GJJ160204), the State Key Laboratory of Food Science and Technology of Nanchang University, China(No.SKLF-ZZA-201612) and the Graduate Student Innovation Program of Nanchang University, China(No.cx2016053)

摘要/Abstract

摘要：

基于重铬酸根离子(Cr₂O^2-₇)对胞苷保护的荧光铜纳米簇(CuNCs)的猝灭作用, 构建了一种可用于检测Cr₂O^2-₇的荧光传感器. 实验结果表明, 该传感体系检测Cr₂ O^2-₇的线性范围为0.05~7.0 μmol/L, 检出限为24 nmol/L(S/N=3). 该传感器对Cr₂ O^2-₇的检测具有良好的选择性, 可用于湖水样中Cr₂ O^2-₇的检测.

关键词: 铜纳米簇, 重铬酸根, 荧光传感器

Abstract:

We constructed a fluorescent sensor for the detection of dichromate anion(Cr₂O^2-₇) on the basis of the quenching effect of Cr₂O^2-₇ on the fluorescence of cytidine-protected copper nanoclusters(CuNCs). The experimental results showed that the Cr₂O^2-₇ sensing system provided a linear range of 0.05—7.0 μmol/L with a low detection limit(S/N=3) of 24 nmol/L. In addition, the sensing system exhibited a good selectivity for Cr₂O^2-₇ detection, and could be applied for Cr₂O^2-₇ detection in lake water samples.

Key words: Copper nanoclusters, Dichromate, Fluorescence sensor

中图分类号:

O657

TrendMD:

陈天霞, 张正涛, 王勇, 倪永年. 胞苷保护的铜纳米簇新型荧光探针检测重铬酸根离子. 高等学校化学学报, 2017, 38(10): 1737.

CHEN Tianxia, ZHANG Zhengtao, WANG Yong, NI Yongnian. Cytidine-protected Copper Nanoclusters as a Novel Fluorescent Probe for the Detection of Dichromate Anion^†. Chem. J. Chinese Universities, 2017, 38(10): 1737.

图/表 6

Scheme 1 CuNCs-based fluorescent detection of Cr2O72-

Fig.1 TEM image(A), FTIR spectrum(B), photostability curve(C) of CuNCs and fluorescence intensity(D) of CuNCs vs. NaCl concentration

Fig.2 UV-Vis absorption spectrum of CuNCs(A) and fluorescence excitation(Ex) and emission(Em) spectra of CuNCs with and without 5.0 μmol/L Cr2O72- and UV-Vis absorption spectra of 5.0 μmol/L Cr2O72-(B)Insets of (B) are the optical images under 365 nm UV light.

Fig.3 Fluorescence emission spectra of CuNCs in the presence of different concentrations of Cr2O72-(A) and the relationship between ΔF and the concentration of Cr2O72- (B)(A) Concentration of CuNCs from top to bottom/(μmol·L-1): 0, 0.01, 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 7.0, 8.0, 10.0, 12.0, 14.0, 16.0, 18.0. Inset of (B) shows the corresponding linear calibration plot.

Fig.4 Fluorescence emission spectra of CuNCs in the presence or absence(blank) of each ion(A) and selectivity of CuNCs for Cr2O72-(B)(A) Concentration of Cr2O72- or any other ion is 1.0 μmol/L. Other ions include Blank, S2-, SCN-, S2O32-, Cl-, NO2-, F-, Br-, ClO3-, IO4-, BrO3-, PO43-, SO32-, H2PO4-, I-, Al3+, Pb3+, Fe3+, Mg2+, Cd2+, Cr3+, Co2+, Ba2+, Mn2+, Ca2+, Fe2+, Ni2+, Hg2+, Zn2+, Cu2+, NH4+, C15H34N+, C8H20N+. (B) ΔF=F0-F; F0 and F denote the fluorescence intensity without(blank) and with each ion, respectively. Ions from left to right: Cr2O72-, blank, S2-, SCN-, S2O32-, Cl-, NO2-, F-, Br-, ClO3-, IO4-, BrO3-, PO43-, SO32-, H2PO4-, I-, Al3+, Pb3+, Fe3+, Mg2+, Cd2+, Cr3+, Co2+, Ba2+, Mn2+, Ca2+, Fe2+, Ni2+, Hg2+, Zn2+, Cu2+, NH4+, C8H20N+, C15H34N+.

Table 1 Determination of Cr2O72- in spiked water samples(n=3)

Sample source	No.	Added/(μmol·L^-1)	Mean found/(μmol·L^-1)	Mean Recovery^*(%)	RSD(%)
Qingshan lake	1	0.50	0.59	118	8.3
	2	1.00	0.96	96	5.6
	3	1.50	1.48	99	2.9
Ganjiang river	1	0.50	0.49	98	3.5
	2	1.00	0.96	96	2.6
	3	1.50	1.62	108	3.9

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胞苷保护的铜纳米簇新型荧光探针检测重铬酸根离子

Cytidine-protected Copper Nanoclusters as a Novel Fluorescent Probe for the Detection of Dichromate Anion^†

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胞苷保护的铜纳米簇新型荧光探针检测重铬酸根离子

Cytidine-protected Copper Nanoclusters as a Novel Fluorescent Probe for the Detection of Dichromate Anion†

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Cytidine-protected Copper Nanoclusters as a Novel Fluorescent Probe for the Detection of Dichromate Anion^†