功能化石墨烯的制备及抗癌药物递送载体的研究

doi:10.7503/cjcu20160430

高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (10): 1900.doi: 10.7503/cjcu20160430

功能化石墨烯的制备及抗癌药物递送载体的研究

罗泽伟¹, 王益民¹, 刘坤平^1,², 魏福静¹, 李玉¹, 段忆翔¹()

1. 四川大学生命科学学院分析仪器研究中心, 生物资源与生态环境教育部重点实验, 成都 610064
2. 成都大学药学与生物工程学院, 成都 610106

收稿日期:2016-06-22 出版日期:2016-10-10 发布日期:2016-09-18
作者简介:联系人简介: 段忆翔, 男, 博士, 教授, 博士生导师, 主要从事活体成像技术和新型生物传感器研究. E-mail:yduan@scu.edu.cn
基金资助:
国家自然科学基金(批准号: 21275105)、中国博士后科学基金(批号: 2013M531961)、国家千人计划和四川省百人计划资助

Preparation of a Functionalized Graphene and Its Role as Delivery Carrier for Anti-cancer Drug^†

LUO Zewei¹, WANG Yimin¹, LIU Kunping^1,², WEI Fujing¹, LI Yu¹, DUAN Yixiang^1,^*()

1.Research Center of Analytical Instrumentation, Key Laboratory of Bio-resource and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China
2. College of Pharmaly and Biological Engineering, Chengdu University, Chengdu 610064, China

Received:2016-06-22 Online:2016-10-10 Published:2016-09-18
Contact: DUAN Yixiang E-mail:yduan@scu.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(No.21275105), the China Postdoctoral Science Foundation(No.2013M531961), the National Recruitment Program of Global Experts and the Hundred Talents Program of Sichuan Province, China

摘要/Abstract

摘要：

以聚苯乙烯磺酸钠(PSS)为保护剂, 利用水合肼还原氧化石墨烯制备了一种新型的聚苯乙烯磺酸钠功能化石墨烯(PSS-GNS). 结果表明制备的PSS-GNS是水溶分散性的纳米片层材料. 考察了PSS-GNS对模型抗癌药物罗丹明6G(R6G)的吸附行为, 结果表明PSS-GNS对R6G吸附量较大(2.77 mg/mg). 体外释放研究结果表明PSS-GNS/R6G对R6G的释放具有pH响应性和缓释作用. PSS-GNS的细胞毒性较低, 能顺利进入癌细胞内并持续缓慢地释放R6G. 因此, PSS-GNS有望成为一种新型的抗癌药物递送载体.

关键词: 功能化石墨烯, 抗癌药物递送载体, 吸附-释放, 细胞毒性

Abstract:

A novel composite of poly(sodium-p-styrenesulfonate) functionalized graphene nanosheets(PSS-GNS) was prepared through reducing graphene oxide(GO) with hydrazine hydrate as the reducing agent and poly(sodium-p-styrenesulfonate)(PSS) as the protective agent. Then, the properties of PSS-GNS were characterized using ultraviolet-visible spectrometer(UV-Vis), Fourier transform infrared spectrometer(FTIR), X-ray diffraction(XRD), Raman spectrometer, scanning electron microscope(SEM), transmission electron microscope(TEM) and atomic force microscope(AFM). The results reveal that PSS-GNS synthesized are in the form of water-soluble and randomly dispersible nanosheets. Furthermore, the absorption behavior of PSS-GNS for rhodamine 6G used as a model anticancer drug was investigated, which demonstrate that PSS-GNS have a high loading capacity of 2.77 mg/mg. Release profiles in vitro indicate that the release of R6G from PSS-GNS/R6G complexes is pH-dependent and slow-release. PSS-GNS also exhibits low cytotoxicity against cancer cells. PSS-GNS/R6G complexes were easily delivered into the cancer cells and sustained the slow release of R6G for a long time. In conclusion, PSS-GNS could be apromising carrier for anti-cancer drug delivery.

Key words: Functionalized graphene, Anticancer drug delivery carrier, Loading and release, Cytotoxicity

中图分类号:

TrendMD:

罗泽伟, 王益民, 刘坤平, 魏福静, 李玉, 段忆翔. 功能化石墨烯的制备及抗癌药物递送载体的研究. 高等学校化学学报, 2016, 37(10): 1900.

LUO Zewei, WANG Yimin, LIU Kunping, WEI Fujing, LI Yu, DUAN Yixiang. Preparation of a Functionalized Graphene and Its Role as Delivery Carrier for Anti-cancer Drug^†. Chem. J. Chinese Universities, 2016, 37(10): 1900.

图/表 7

Fig.1 FTIR(A) and UV-Vis(B) spectra of PSS(a), GO(b) and PSS-GNS(c)

Fig.2 XRD patterns of natural flake graphite(a) and PSS-GNS(b)(A) and Raman spectra of natural flake graphite(a) and PSS-GNS(b)(B)

Fig.3 SEM(A), TEM(B) and AFM(C) images and height profiles(D) of PSS-GNS

Fig.4 Linear curve of the fluorescence intensity to R6G in different concentrations(A) and fluorescence spectra of R6G loaded on PSS-GNS in different volume(B) (A) The inset describes the corresponding fluorescence spectra of R6G in different concentrations.

Fig.5 Time dependent R6G releasing profiles from PSS-GNS/R6G complexes in pH=7.4(a), pH=4.6(b), pH=2.0(c) buffer(A) and release profiles of R6G from PSS-GNS/R6G complexes in water(a) and ethanol(b) for 2 h(B)

Fig.6 Viability of MDA-MB-231 cancer cells treated with different concentrations of PSS-GNS

Fig.7 Confocal laser scanning microscopy fluorescence images of MDA-MB-231 cancer cells treated with PSS-GNS/R6G for different time The bar is 20.0 μm. (A1—A6) R6G; (B1—B6) Hoechst; (C1—C6) bright field; (D1—D6) merger. Time: (A1—D1) 15 min; (A2—D2) 30 min; (A3—D3) 1 h; (A4—D4) 4 h; (A5—D5) 12 h; (A6—D6) 24 h.

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功能化石墨烯的制备及抗癌药物递送载体的研究

Preparation of a Functionalized Graphene and Its Role as Delivery Carrier for Anti-cancer Drug^†

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功能化石墨烯的制备及抗癌药物递送载体的研究

Preparation of a Functionalized Graphene and Its Role as Delivery Carrier for Anti-cancer Drug†

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Preparation of a Functionalized Graphene and Its Role as Delivery Carrier for Anti-cancer Drug^†