血红蛋白和SiO2凝胶层层组装膜在碳纳米管/金纳米粒子修饰电极界面上的直接电化学及电催化研究

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (10): 2293.

血红蛋白和SiO2凝胶层层组装膜在碳纳米管/金纳米粒子修饰电极界面上的直接电化学及电催化研究

徐颖¹,张小燕¹,杨静²,何品刚¹,方禹之¹

1. 华东师范大学化学系, 上海 200062;
2. 东华大学环境科学与工程学院, 上海 201620

收稿日期:2010-11-03 修回日期:2010-12-06 出版日期:2011-10-10 发布日期:2011-09-11
通讯作者: 何品刚;杨静 E-mail:pghe@chem.ecnu.edu.cn;jingyang@dhu.edu.cn
基金资助:
上海市教育委员会和上海市教育发展基金会晨光计划(批准号: 2008CG30)和东华大学青年教师科研启动基金项目(批准号: 113100044039)资助.

Electrochemistry and Electrocatalysis of Hemoglobin with SiO2 Gel Film Layer-by-layer Assembled on Carbon Nanotubes-Au Nanoparticles Modified Electrode

XU Ying¹, ZHANG Xiao-Yan¹, YANG Jing^2*, HE Pin-Gang^1*, FANG Yu-Zhi¹

1. Department of Chemistry, East China Normal University, Shanghai 200062, China;
2. School of Environmental Science and Engineering, Donghua University, Shanghai 201620, China

Received:2010-11-03 Revised:2010-12-06 Online:2011-10-10 Published:2011-09-11
Contact: HE Pin-Gang;YANG Jing E-mail:pghe@chem.ecnu.edu.cn;jingyang@dhu.edu.cn
Supported by:
上海市教育委员会和上海市教育发展基金会晨光计划(批准号: 2008CG30)和东华大学青年教师科研启动基金项目(批准号: 113100044039)资助.

摘要/Abstract

摘要： 以SiO2凝胶膜和蛋白质交互组装法固定血红蛋白(Hb), 对其进行了电化学和电催化研究. 首先制备碳纳米管/金纳米粒子复合材料修饰的MWNTs-Au/GC电极, 为防止蛋白质在电极表面流失, 将Hb和自制的SiO2凝胶膜交替滴涂到电极表面, 得到SiO2/Hb层层组装膜修饰电极, 即{SiO2/Hb}n/MWNTs-Au/GC电极, n=2为优化层数. Hb在{SiO2/Hb}2/MWNTs-Au/GC电极上仍能保持其特有的生物活性, 并能与电极进行稳定快速的电子直接转移, 同时表现出过氧化物酶特性, 对H2O2具有良好的生物电催化还原能力.

关键词: 血红蛋白, 碳纳米管, 金纳米粒子, SiO2凝胶

Abstract: Protein direct electrochemistry study and its electro\|catalysis analysis were introduced, involving layer-by-layer alternate assembly of hemoglobin(Hb)and SiO2 gel film onto electrode surface. The electrode was firstly modified with the nano-compound of multi-walled carbon nanotubes and Au nanoparticles, i.e., MWNTs-Au/GC electrode. Then Hb and SiO2 gel film were alternately spread onto the electrode surface to stabilize the protein and its amount and bioactivity, thus resulting {SiO2/Hb}n/MWNTs-Au/GC electrode. Under the optimized n=2, the Hb on the {SiO2/Hb}2/MWNTs-Au/GC electrode displayed its intrinsic bioactivity well, transferring electrodes with electrode and catalyzing H2O2 reduction as one peroxidaze enzyme.

Key words: Hemoglobin, Carbon nanotube, Au nanoparticle, SiO2 gel

中图分类号:

O657.1

TrendMD:

徐颖张小燕杨静何品刚方禹之. 血红蛋白和SiO2凝胶层层组装膜在碳纳米管/金纳米粒子修饰电极界面上的直接电化学及电催化研究. 高等学校化学学报, 2011, 32(10): 2293.

XU Ying, ZHANG Xiao-Yan, YANG Jing*, HE Pin-Gang*, FANG Yu-Zhi. Electrochemistry and Electrocatalysis of Hemoglobin with SiO2 Gel Film Layer-by-layer Assembled on Carbon Nanotubes-Au Nanoparticles Modified Electrode. Chem. J. Chinese Universities, 2011, 32(10): 2293.

参考文献

[1] Milazzo G., Blank M.. Bioelectro chemistry and Bioenergetics（生物电化学-生物氧化还原反应） [M]. Translated by XIAO Ke（肖科）, TANG Bao-zhang（唐宝璋）, SHI Er-gang（史尔纲）. Tianjin: Tianjin Science Press, 1990 [2] JU Huang-Xian（鞠熀先）. Electrochemistry and Biosensors (电分析化学与生物传感技术) [M], Beijing: Science Press, 2006 [3] LUO Guo-An（罗国安）, WANG Zong-Hua（王宗花）, WANG Yi-Ming（王义明）. Fabrication and Application of Biocompatible Electrode（生物兼容性电极购置及应用）[M], Beijing: Science Press, 2006 [4] Bowden E. F., Hawkridge F. M., Blount H. N.. Bioelectrochem. Bioenerg. [J], 1980, 7 (3): 447—457 [5] Serre P. A., Haladjian J., Bianco P. J.. Electroanal. Chem. [J], 1981, 122: 327—336 [6] Nicholson R. S., Shain I.. Anal. Chem. [J], 1964, 36: 707—723 [7] DONG Shao-jun（董绍俊），CHE Guang-li（车广礼），XIE Yuan-wu（谢远武）. Chemially Modified Electrodes （化学修饰电极） [M], Beijing: Science Press, 1995 [8] Shao S., Clark P. A., Bowden E. F., et al.. J. Phys. Chem. [J], 1993, 97: 6564—6572 [9] Bianco P., Haladjian J.. Electro. Chim. Acta. [J], 1997, 142(4): 587—594 [10] Niu J. J., Guo Y. Z., Dong S. J.. J. Electroanal. Chem. [J], 1995, 399: 41—46 [11] Rusling J. F., Accounts Chem. Res. [J], 1998, 31(6): 363—369 [12] Davis J. J., Green M. L. H., Hill H. A. O., et al.. Inorg. Chim. Acta. [J], 1998, 272 (1-2): 261—266 [13] Yang J., Xu Y., Zhang R. Y., et al.. Electroanal. [J], 2009, 21: 1672—1677 [14] TANG Fang-Qiong（唐芳琼）, WEI Zheng（韦正）, CHEN Dong（陈东）. et al.. Chem. J. Chinese University（高等学校化学学报）[J], 2000, 20(1): 91—94 [15] ZHANG Juan（张娟）, XU Jing-Juan（徐静娟）, CHEN Hong-Yuan（陈洪渊）. Chem. J. Chinese University（高等学校化学学报）[J], 2004, 25(4): 614—617 [16] Armstrong F. A., Wilson G. S.. Electrochim. Acta. [J], 2000, 45(15-16): 2623—2645 [17] Doron A., Katz E., Willner I.. Langmuir [J], 1995, 11: 1313—1317 [18] Braun S., Rappoport S., Zueman R.. Matter. Lett. [J], 1990, 10: 1—5 [19] LUO Guo-An（罗国安）, WANG Zong-Hua（王宗花）, WANG Yi-Ming（王义明）. Construction and Application of Bio-electrodes（生物兼容性电极购置及应用） [M], Beijing: Science Press, 2006, 32—44 [20] Kioul A., Mascia L., Non-Cryst J.. Solids [J], 1995, 175: 169—186 [21] Rusling J. S., Kumosinski T. F., Nonlinear Computer Modeling of Chemical and Biochemical Data [M], San Diego: Academic Press, 1996, 117—134 [22] Dong A., Huang P., Caughey W. S.. Biochemistry [J], 1990, 29: 3303—3308 [23] Dujardin E., Ebbesen T. W., Hiura H., et al.. Science [J], 1994, 265:1850—1852 [24] Xu Ying,?Yang Lin,?He Pin-gang,?et al.. J. Biomed. Nanotechnol. [J], 2005, 1: 202—207 [25] Murry R W.; Eds: Bard A. J.. Electroanalytical Chemistry, Vol. 13 [M], New York: Mercel Dekker, 1984, 191—368 [26]Feng J. J., Zhao G., Xu J. J., et al.. Anal. Biochem. 2005, 342: 280—286 [27] LUO Guo-An（罗国安）, WANG Zong-Hua（王宗花）, WANG Yi-Ming（王义明）. Fabrication and Application of Biocompatible Electrode（生物兼容性电极购置及应用） [M], Beijing: Science Press, 2006, 277—305 [28] Armstrong F. A.. Biochem. Soc. Trans. [J], 1999, 27(2): 206—210 [29] Laviron E.. J. Electroanal. Chem. [J],?1979, 100: 263—270 [30] Laviron E.. J. Electroanal. Chem.?[J], 1979, 101: 19—28 [31] Zhao Y. D., Bi Y. H., Zhang W. D., et al.. Talanta [J], 2005, 65: 489—494 [32] Shi G. Y., Sun Z. Y., Liu M. C., et al.. Anal. Chem. [J], 2007, 79: 3581—3588 [33] Wade R. S., Castro C. E.. J. Am. Chem. Soc. [J], 1973, 95: 231—234 [34] L?tzbeyer T., Schuhmann W., Schmidt H. L.. Bioelectrochem. Bioenerg. [J], 1997, 42: 1—6 [35] Kamin R. A., Wilson G. S.. Anal. Chem. [J], 1980, 52: 1198—1205 [36] Wang J., Musameh M., Lin Y.. J. Am. Chem. Soc. [J], 2003, 125: 2408—2409 [37] Maduraiveeran G., Ramaraj R.. J. Electroanal. Chem. [J], 2007, 608: 52—58 [38] Xiao H. Y., Lu H. X., Chen H. Y.. Anal. Biochem. [J], 2000, 278: 22—28 [39] Kamin R. A., Wilson G. S.. Anal. Chem. [J], 1980, 52: 1198—1205 [40] LI Gen-xi（李根喜）, ZHU De-xu（朱德煦）, Electrochemistry Technologies for Biological Research（探究生命活动的电化学技术）www.lifescience.net.cn/read.asp?FileID=2007425143933