黄芩素和黄芩苷与人血清白蛋白相互作用的光谱研究

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (9): 1834.

黄芩素和黄芩苷与人血清白蛋白相互作用的光谱研究

张巍¹, 赵雨², 白雪媛², 惠歌¹, 赵大庆², 赵冰¹

1. 吉林大学超分子结构与材料国家重点实验室, 长春 130012;
2. 长春中医药大学新药研发中心, 长春 130117

收稿日期:2010-01-21 出版日期:2010-09-10 发布日期:2010-09-10
通讯作者: 赵冰, 男, 博士, 教授, 博士生导师, 主要从事光谱分析研究. E-mail: zhaob@mail.jlu.edu.cn
基金资助:
国家自然科学基金(批准号: 20921003, 20973034)、国家科技支撑计划(批准号: 2007BAI38B03)和全国高校学科创新引智工程(批准号: B06009)资助.

Studies on the Interaction for Baicalein and Baicalin with Human Serum Albumin by Spectroscopic Methods

ZHANG Wei¹, ZHAO Yu², BAI Xue-Yuan², HUI Ge¹, ZHAO Da-Qing², ZHAO Bing^1*

1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China;
2. Center for New Drugs Research, Changchun University of Traditional Chinese Medicine, Changchun 130117, China

Received:2010-01-21 Online:2010-09-10 Published:2010-09-10
Contact: ZHAO Bing. E-mail: zhaob@mail.jlu.edu.cn
Supported by:
国家自然科学基金(批准号: 20921003, 20973034)、国家科技支撑计划(批准号: 2007BAI38B03)和全国高校学科创新引智工程(批准号: B06009)资助.

摘要/Abstract

摘要： 采用荧光光谱、紫外-可见光谱和表面增强拉曼光谱研究了黄芩素和黄芩苷与人血清白蛋白的相互作用, 得到了分子间的结合状态和吸附形态方面的信息. 通过比较黄芩素和黄芩苷的普通拉曼和表面增强拉曼光谱, 确立了黄芩素和黄芩苷的正常构象, 同时考察了结合上人血清白蛋白后不同浓度黄芩素和黄芩苷的吸附方式差别.

关键词: 黄芩素, 黄芩苷, 人血清白蛋白, 荧光光谱, 紫外-可见光谱, 表面增强拉曼光谱

Abstract: The fluorescence, ultraviolet-visible and surface-enhanced Raman scattering spectroscopy were applied to study the interaction between baicalein/baicalin and human serum albumin, as the subsequent consequence, the information of intermolecular adsorption and surface-enhanced Raman Scattering spectroscopy, the normal crientation were obtained. By the comparison of norml Ramn and onformation of baicalein and baicalin were confirmed. Besides that, we also studied the orientated difference between baicalein and baicalin after their combining to human serum albumin. In addition, the state of freedom ions on the binding of baialein/baicalin and human serum were also investigated in physiological conditions, which is the most accessible work of simulation in vitro.

Key words: Baicalein, Baicalin, Human serum albumin, Fluorescence spectrum, Ultraviolet-visible spectrum, Surface enhanced Raman scattering spectroscopy

TrendMD:

张巍, 赵雨, 白雪媛, 惠歌, 赵大庆, 赵冰1. 黄芩素和黄芩苷与人血清白蛋白相互作用的光谱研究. 高等学校化学学报, 2010, 31(9): 1834.

ZHANG Wei, ZHAO Yu, BAI Xue-Yuan, HUI Ge, ZHAO Da-Qing, ZHAO Bing*. Studies on the Interaction for Baicalein and Baicalin with Human Serum Albumin by Spectroscopic Methods. Chem. J. Chinese Universities, 2010, 31(9): 1834.

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