环氧基修饰周期性介孔有机氧化硅对漆酶的固定化作用

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (8): 1579.

环氧基修饰周期性介孔有机氧化硅对漆酶的固定化作用

兰甲宁, 纳薇, 韦奇, 李群艳, 王为, 聂祚仁

北京工业大学材料科学与工程学院, 北京 100124

收稿日期:2009-11-06 出版日期:2010-08-10 发布日期:2010-08-10
通讯作者: 韦奇, 男, 博士, 教授, 主要从事多孔材料的研究. E-mail: qiwei@bjut.edu.cn
基金资助:
国家杰出青年科学基金(批准号: 50525413)和北京市属高等学校人才强教深化计划资助.

Immobilization of Laccase on Epoxy Group-functionalized Periodic Mesoporous Organosilicas

LAN Jia-Ning, NA Wei, WEI Qi*, Li Qun-Yan, WANG Wei, NIE Zuo-Ren

College of Materials Science & Engineering, Beijing University of Technology, Beijing 100124, China

Received:2009-11-06 Online:2010-08-10 Published:2010-08-10
Contact: WEI Qi. E-mail: qiwei@bjut.edu.cn
Supported by:
国家杰出青年科学基金(批准号: 50525413)和北京市属高等学校人才强教深化计划资助.

摘要/Abstract

摘要： 以三嵌段共聚物P123为模板剂, 在酸性条件下通过1,2-三乙氧基硅基乙烷(BTESE)和3-含氧缩水甘油基-丙基-三甲氧基硅烷(GPTMS)共水解缩聚合成环氧基修饰的周期性介孔氧化硅(PMOs), 以修饰后的PMOs为载体对漆酶进行固定化, 研究了环氧基修饰对固定化酶稳定性的影响. 通过XRD、TEM、固态NMR和N₂吸附等手段表征材料的修饰效果、孔结构以及漆酶的固定化. 结果表明, 修饰后的材料保持良好的孔结构, 环氧基的修饰有利于提高固定化酶的活力, 基于环氧基修饰PMOs的固定化酶具有较高稳定性.

关键词: 周期性介孔氧化硅, 环氧基, 漆酶, 固定化, 稳定性

Abstract: Epoxy group-functionalized periodic mesoporous organosilicas(FPMOs) was synthesized by the co-condensation of 1,2-bis(triethoxysilyl)ethane(BTESE) and 3-glycidoxypropyltrimethoxylsilane(GPTMS) in the presence of triblock copolymer poly(ethylene glycol)-b-poly(propylene glycol)-b-poly(ethylene glycol)(P123) under acidic conditions and utilized as a support for laccase immobilization. The effect of epoxy group-functionalization on the immobilization of laccase and the stability of immobilized laccase were investigated in detail. The level of functionalization, the pore structure and the laccase immobilization were detected by means of solid-state NMR, N2 adsorption and UV-Vis, respectively. It is revealed that epoxy groups are successfully attached covalently to the pore wall of PMOs and the functionalized materials retain a desirable pore structure. Laccase immobilized on functionalized FPMOs exhibits a higher activity and stability than that adsorbed on pure PMOs.

Key words: Periodic mesoporous organosilica, Epoxy group, Laccase, Immobilization, Stability

TrendMD:

兰甲宁, 纳薇, 韦奇, 李群艳, 王为, 聂祚仁. 环氧基修饰周期性介孔有机氧化硅对漆酶的固定化作用. 高等学校化学学报, 2010, 31(8): 1579.

LAN Jia-Ning, NA Wei, WEI Qi*, Li Qun-Yan, WANG Wei, NIE Zuo-Ren. Immobilization of Laccase on Epoxy Group-functionalized Periodic Mesoporous Organosilicas. Chem. J. Chinese Universities, 2010, 31(8): 1579.

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