高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

交联结构对肝素/壳聚糖层层组装多层膜内皮细胞相容性的影响

侯悦, 林全愧, 计剑, 沈家骢   

  1. 浙江大学高分子科学与工程学系, 教育部高分子合成与功能构造重点实验室, 杭州 310027
  • 收稿日期:2008-03-10 修回日期:1900-01-01 出版日期:2008-09-10 发布日期:2008-09-10
  • 通讯作者: 计剑

Improvement of Endothelialization of(Heparin/Chitosan) Multilayer Films via Chemical Cross-Linking

HOU Yue, LIN Quan-Kui, JI Jian*, SHEN Jia-Cong   

  1. Key Laboratory of Macromolecule Synthesis and Functionalzation of Minster of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
  • Received:2008-03-10 Revised:1900-01-01 Online:2008-09-10 Published:2008-09-10
  • Contact: JI Jian

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被引次数

9

摘要： 采用1-乙基-(3-二甲基氨基丙基)碳酰二亚胺交联技术对具有抗凝血抗菌作用的肝素/壳聚糖多层膜进行交联, 研究了交联结构对多层膜稳定性和血管内皮细胞亲和性的影响. QCM-D结果显示, 交联可有效地提高多层膜的稳定性, 在模拟人体血液流速(3.0 cm/s)下保持良好的稳定. 体外内皮细胞的研究结果显示, 多层膜的交联可有效地调节肝素/壳聚糖多层膜表面粘弹性, 并显著增加内皮细胞的粘附与生长. 交联的肝素/壳聚糖多层膜有望成为理想的心血管功能界面涂层材料.

关键词: 肝素, 壳聚糖, 内皮细胞, 交联, 层层自组装

Abstract: Heparin(HEP) /chitosan(CHI) multilyayer films were cross-linked by 1-ethyl-3-(3-dimethy-laminopropyl) carbodiimide(EDC) technique. The stability and endothelial cell activities of multilayer films were investigated. QCM-D test indicates that the cross-linking could improve the stablility of the multilayer films. The multilayer films had good stability when simulating human blood flow rate-3.0 cm/s. In vitro endothelial cell tests suggest that cross-linking could effectively modify the viscoelasticity of(HEP/CHI) multilayer film surface and improve the adhesion and proliferation behavior of endothelial cells. The cross-linked(HEP/CHI) multilayered film might have a great potential as a coating for cardiovascular materials.

Key words: Heparin, Chitosan, Endothelial cell, Cross-linking, Layer-by-layer self-assembly

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