高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

茶多酚锰合成、表征及络合和诱导肿瘤细胞凋亡的研究

黄河宁1,3, 李安章1, 翁露娜1, 林庆梅2, 黄河清1,2, 郑忠辉1, 李棋福1, 蔡宗苇4   

    1. 厦门大学生命科学学院, 细胞生物学与肿瘤细胞工程教育部重点实验室,
    2. 海洋与环境学院环境科学研究中心, 厦门361005;
    3. 三明学院化学与生物工程系, 三明 365004; 4
    4. 香港浸会大学化学系, 香港
  • 收稿日期:2006-06-19 修回日期:1900-01-01 出版日期:2007-06-10 发布日期:2007-06-10
  • 通讯作者: 黄河清

Synthesis, Characterization and Binding of Tea Polyphenol-Manganese and Apoptosis Rate of Induced Tumor Cells

HUANG He-Ning1,3, LI An-Zhang1, WENG Lu-Na1, LIN Qing-Mei2, HUANG He-Qing1,2*, ZHENG Zhong-Hui1, LI Qi-Fu1, CAI Zong-Wei4   

    1. The Key Laboratory of MOE for Cell Biology and Tumor Cell Engineering, School of Life Sciences,
    2. College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China;
    3. Department of Chemical and Biological Engineering, College of Sanming, Sanming 365004, China;
    4. Department of Chemistry, Hong Kong Baptist University, Hong Kong
  • Received:2006-06-19 Revised:1900-01-01 Online:2007-06-10 Published:2007-06-10
  • Contact: HUANG He-Qing

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被引次数

31

摘要： 合成了茶多酚锰(TP-Mn)和茶多酚锗(TP-Ge), 并采用红外光谱法及液相色谱质谱联用技术表征TP-Mn的理化特性. 选用反向液相色谱(RP-HPLC)和电感藕合等离子体质谱(ICP-MS)技术研究牛血清白蛋白(BSA)络合TP-Mn的能力, 指出BSA可直接络合于TP-Mn. 采用MTT法研究TP和TP-Mn诱导Hela卵巢癌细胞的凋亡率. 结果表明, TP和TP-Mn均能诱导Hela卵巢癌细胞凋亡, 但TP-Mn诱导凋亡率约为TP的2倍. 比较TP-Ge和TP-Mn诱导Raji人B淋巴瘤细胞凋亡率发现, 两者诱导肿瘤细胞的凋亡率几乎相同, 均高达86%左右. 人血清白蛋白(HSB)可作为输送TP-Ge和TP-Mn的药物载体.

关键词: 茶多酚锰, 茶多酚锗, 肿瘤细胞, 凋亡率, 电感藕合等离子体质谱

Abstract: Both tea polyphenol-manganese(TP-Mn) and germanium(TP-Ge) were synthesized with organic synthesis approaches. The characterizations of TP-Mn were clearly revealed via infrared spectrometry and HPLC-ESI-Q-TOF mass spectrometry, respectively. Both HPLC and ICP-MS methods were employed to study the capacities for bovine serum albumen(BSA) binding to TP-Mn, indicating these capacities feasibility. Apoptosis rates in Hela ovarian induced with TP and TP-Mn were studied by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenly tetrazolium bromide(MTT) method. The experimental results show that both TP and TP-Mn can induced the cancer cell apoptosis, but the apoptosis rate with TP-Mn showed two times higher than that with TP. As compared experiments, the same apoptosis rates, going with up to approximately 86%, in Raji human B lymphoma induced with both TP and TP-Mn were observed directly. Human serum albumen might be employed to be a pharmacy carrier for transferring TP-Mn and TP-Ge in vivo.

Key words: Tea polyphenol-Mn, Tea polyphenol-Ge, Tumor cell, Apoptosis rate, ICP-MS

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