高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

富硒灵芝中一种新含硒蛋白的纯化、性质及其自由基清除活性研究

杜明1,2, 赵镭1, 李朝睿1, 赵广华1, 胡小松1   

    1. 中国农业大学食品科学与营养工程学院, 北京 100083;
    2. 哈尔滨工业大学食品科学与工程学院, 哈尔滨 150001
  • 收稿日期:2006-02-10 修回日期:1900-01-01 出版日期:2007-01-10 发布日期:2007-01-10
  • 通讯作者: 赵广华

Purification and Properties of a Novel Se-containing Protein from Se-enriched Ganoderma lucidum and Its Antioxidant Activity

DU Ming1,2, ZHAO Lei1, LI Chao-Rui1, ZHAO Guang-Hua1, HU Xiao-Song1   

    1. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China;
    2. College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150001, China
  • Received:2006-02-10 Revised:1900-01-01 Online:2007-01-10 Published:2007-01-10

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被引次数

38

摘要： 通过硫酸铵沉淀、离子交换层析和分子排阻层析等方法, 从富硒灵芝中获得了一种新的含硒蛋白, 命名为Se-GL-P, 并研究了此蛋白的性质、抗氧化活性与其硒含量间的关系. 结果表明, 此蛋白的分子量为36600, 分子中约含有19.8%的糖链, N端的氨基酸残基序列为DINGGGATLPQKLYLTPDVL, 属于DING蛋白家族. 硒含量为4.87 mg/g, 具有较高的羟自由基和超氧自由基清除活性. 研究发现, Se-GL-P的抗氧化活性的提高与其中硒含量的提高相关.

关键词: 富硒灵芝, 硒, 含硒蛋白, 抗氧化活性

Abstract:

A novel water-soluble fungi Se-containing protein, named as Se-GL-P, was purified for the first time from Se-enriched GL to establish the relationship between the antioxidant activity of protein and its Se content. This protein was isolated and purified to homogeneity by using ammonium sulfate precipitation followed by two consecutive anion exchange chromatography and size exclusion chromatography. This protein in its native state was identified as a monomer of 36600 estimated by 19.8% weight carbohydrate. The protein is acidic and the N-terminal shows a sequence of DINGGGATLPQKLYLTPDVL, suggesting that this protein belongs to a family of DING protein. The Se content of this protein is 4.87 mg/g protein, and the protein has stronger scavenging activities on superoxide and hydroxyl radicals. It is found that the increasing antioxidant property of this protein depends quantitatively on the increase of its Se content.

Key words: Se-enriched Ganoderma lucidum, Selenium, Se-containing protein, Antioxidant activity

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