高等学校化学学报

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (1): 5.

• 研究论文 • 上一篇    下一篇


固定化无机结合肽AG4(NPSSLFRYLPSD)仿生制备银微晶体

张旭1, 陈劲春1, 杨鹏2, 杨万泰2   

  1. 1. 北京化工大学生命科技学院; 2. 北京化工大学材料科学与工程学院, 北京 100029
  • 收稿日期:2005-01-18 出版日期:2006-01-10 发布日期:2006-01-10
  • 通讯作者: 陈劲春(1947年出生)， 男， 博士， 教授， 博士生导师， 主要从事基因工程和分子仿生研究. E-mail: jingchunchen@hotmail.com
  • 基金资助:

    国家自然科学基金(批准号： 50433040)资助.

Silver Biomimetic Mineralization in vitro with Inorganicbinding  Peptide AG4(NPSSLFRYLPSD) Immobilized on PET Film

ZHANG Xu1, CHEN JinChun1, YANG Peng2, YANG WanTai2   

  1. 1. College of Life Science and Technology, 2. College of Material Science and Engineering,
    Beijing University of Chemical Technology, Beijing 100029, China)
  • Received:2005-01-18 Online:2006-01-10 Published:2006-01-10
  • Contact: CHEN JinChun,E-mail: jingchunchen@hotmail.com

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摘要：

摘要利用固定化的AG4进行了离体仿生制备银微晶的研究. 受静电力推动, 正离子化的PET-N(CH3)2膜表面吸附了AG4, AG4/ PET-N+(CH3)2膜经AgNO3溶液浸泡后, 用SEM观测发现了大小为1~2 μm的银微晶体, 其多数呈三角形和正方形. 经XPS检测, 银微晶体的Ag3d结合能为368.1 eV(Ag0的Ag3d结合能标准值为368.2 eV), 同时还测得硝酸银的Ag3d结合能为368.6 eV. 由此推断， 固定化后的AG4在离体条件下能将AgNO3溶液中的Ag+还原为Ag0. 对Ag+在AG4/ PET-N(CH3)2膜上还原并形成微晶体的机理进行了初步分析和探讨.

关键词: 无机结合肽； PET膜； 银微晶； 分子仿生

Abstract:

AbstractAG4(NPSSLFRYLPSD)is a peptide that specifically and selectively binds to silver resulting from Dr. Stony′s phage display peptide combination library. The peptides that have similar characteristic are defined as inorganicbinding peptides. In our experiment, AG4 was adsorbed on the surface of protonated PET-N(CH3)2 film for studying its biomineralization behavior in vitro. Based on the XPS results of both silver crystallite on AG4/PET-N+(CH3)2 film and AgNO3 salt, we approved that Ag+ exactly reduced to Ag0 and further formed silver crystallites. In addition, the images of SEM revealed that silver crystallite was 1-2 μm in size with a square shape and a triangular shape. At last, we discussed the reaction mechanism possibly occurring in this process.

Key words: Inorganicbinding peptides; PET film; Silver Micro-crystallite; Molecular biomimetics

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