高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (9): 1640.

• 研究论文 • 上一篇    下一篇

肺鳞癌和小细胞肺癌组织比较蛋白质组学研究

罗国安, 邓斌, 叶能胜, 王义明   

  1. 清华大学生命有机磷化学及化学生物学教育部重点实验室, 北京100084
  • 收稿日期:2004-07-13 出版日期:2005-09-10 发布日期:2005-09-10
  • 基金资助:

    国家自然科学基金重点项目(批准号:90209005);国家自然科学基金(批准号:30271663)资助.

Comparative Proteome Analysis of Human Squamous Lung Carcinomas and Small Cell Lung Carcinomas

LUO Guo-An, DENG Bin, YE Neng-Sheng, WANG Yi-Ming   

  1. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Tsinghua University, Beijing 100084, China
  • Received:2004-07-13 Online:2005-09-10 Published:2005-09-10

PDF (PC)

被引次数

39

摘要： 通过16例人肺鳞癌和小细胞肺癌组织中表达蛋白的二维电泳分离和质谱分析,经数据库检索鉴定了53个蛋白,其中24个蛋白与肺癌发病机制相关,4个蛋白在其它癌症中有报道.表达呈现差异的蛋白点有44个,其中34个在表达量上有差异,10个蛋白在鳞癌和小细胞癌间表现为有和无的关系.蛋白功能分析提示人肺鳞癌与小细胞癌的蛋白质组表达存在差异,分析这些差异蛋白有利于肺癌分型及其生物标志物研究.

关键词: 肺鳞癌, 小细胞肺癌, 蛋白质组学

Abstract: We employed 2-DE with matrix-assisted laser desorption/ionization mass spectrometry(MALDI-TOF-MS) analysis after in-gel digestion protocol to identify the expressing proteins of human squamous lung carcinomas(SQCLC) and small cell lung carcinomas(SCLC) from surgical resection of 16 lung tumor patients. From a total of 59 spots excised from the 2-DE gel, 53 proteins had matches returned from the database search leading to the identification of these proteins. Among them only about 10 proteins have a significant difference(7 for SQCLC and 3 for SCLC, respectively) between SQCLC and SCLC. Meanwhile, 34 proteins, which highly existed in both carcinomas with different expression levels, as well as 6 proteins with almost the same expression levels, were found as the common proteins for both carcinomas. Among these identified proteins, 24 proteins had been previously proposed as the associated proteins with lung carcinomas, and 4 proteins had been linked to the other human carcinomas but not proposed in lung carcinomas.

Key words: Squamous cell lung carcinomas, Small cell lung carcinomas, Proteomics

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