高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (10): 1812-1816.

• 研究论文 • 上一篇    下一篇

新型DNA电化学传感器的研制及其用于DNA氧化性损伤检测的研究

王桂香, 潘芊秀, 王怀生   

  1. 聊城大学化学化工学院, 聊城252059
  • 收稿日期:2004-11-22 出版日期:2005-10-10 发布日期:2005-10-10
  • 基金资助:

    国家自然科学基金(批准号:20475024)资助

Preparation of a Novel DNA Electrochemical Sensor and Its Application to Detection of DNA Oxidative Damage

WANG Gui-Xiang, PAN Qian-Xiu, WANG Huai-Sheng   

  1. College of Chemistry and Chemical Engineering,Liaocheng University,Liaocheng 252059,China
  • Received:2004-11-22 Online:2005-10-10 Published:2005-10-10

摘要: 用溶胶-凝胶法在玻碳电极上制备了纳米多孔羟基磷灰石(HAp)-聚乙烯醇(PVA)涂层膜固定双链DNA,得到了一种新型DNA电化学传感器,检测了由Fenton反应引起的DNA氧化性损伤.结果表明,一定量浓度的抗坏血酸(AA)能加速Fenton反应的进行,使DNA损伤很快达到极限;损伤试剂中Fe2+的浓度越大,产生的羟基自由基(OH.)越多,对DNA的损伤就越严重;损伤试剂中EDTA的浓度越小,溶液中游离的Fe2+以及与DNA键合的Fe2+的浓度则相对越大,对DNA的损伤也就越严重.

关键词: DNA电化学传感器, DNA损伤检测, 羟基磷灰石, Fenton反应

Abstract: A novel DNA electrochemical sensor was prepared by immobilizing dsDNA on the hydroxyapatite(HAp)-polyvinyl alcohol(PVA) coating which was prepared by sol-gel methold on the glass carbon electrode surface.The sensor was used to detect the oxidative damage to dsDNA mediated by Fe2+ Fenton reaction.The results clearly indicate that the DNA damage can be accelerated by the presence of excessive AA in this experiment.The lower concentration of EDTA and the relative higer concentration of Fe2+ in the Fenton reaction solution can produce more OH·,and then result in the deeper damage of DNA.

Key words: DNA electrochemical sensor, Detection of DNA damage, Hydroxyapatite, Fenton reaction

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