高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (11): 2134-2136.

• 研究简报 • 上一篇    下一篇

N-羟乙基-1,8-萘二酰亚胺探针与核苷间电子转移的激光闪光光解研究

李慧卿1, 江致勤1, 王欣1, 潘洋2, 王峰2, 俞书勤2   

  1. 1. 同济大学化学系, 上海200092;
    2. 中国科技大学化学物理系, 合肥230026
  • 收稿日期:2003-08-21 出版日期:2004-11-24 发布日期:2004-11-24
  • 基金资助:

    国家自然科学基金(批准号:20272042,20273066)资助

Electron Transfer Laser Flash Photolysis Between Nucleosides and Probe Triplet N-(2’-Hydroxyethyl)-1,8-naphthalimide

LI Hui-Qing1, JIANG Zhi-Qin1, Wang Xin1, PAN Yang2, WANG Feng2, YU Shu-Qin2   

  1. 1. Department of Chemistry, Tongji University, Shanghai 200092, China;
    2. Department of Physical Chemistry, University of Science and Technology of China, Hefei 230026, China
  • Received:2003-08-21 Online:2004-11-24 Published:2004-11-24

关键词: 萘二酰亚胺, 核苷, 2-脱氧鸟苷, 电子转移, 激光闪光光解

Abstract: The time resolved transient absorption property, kinetics and mechanism of electron transfer(ET) process of the fluorescence probe, N-(2-hydroxyethyl)-1,8-naphthalimide(NPI) were studied. The transient absorption spectra of NPI in H2O-CH3CN solution were successfully detected upon pulsed excitation at 355 nm. 3NPI was proved as the primary excited state with lifetime of 147 μs. 3NPI underwent the T-T energy transfer with β-carotene and ET with triphenyl amine to produce NPI. In the absence of quenchers, the self-quenching of 3NPI by the ground states of NPI took place via ET. The ET from nucleosides to 3NPI was also explored. NPI showed a strong electron-accepting capability and the absorbance of 3NPI was strongly quenched by the nucleosides. The formation of NPI was facilitated to a great extent simultaneously. The bimolecular quenching rate constants of NPI by GUA and DGUA were determined as k3=8.8×106mol-1·L·s-1, k4=2.1×107 mol·L·s-1. The ET between NPI and nucleosides was verified and the mechanism was proposed.

Key words: Naphthalimide, Nucleoside, 2’-Dioxyguanosine, Electron transfer, Laser flash photolysis

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