高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (5): 900.

• 研究快报 • 上一篇    下一篇

SERS标记纳米粒子用于免疫识别

徐抒平1, 王连英2, 徐蔚青1, 赵冰1, 袁航3, 马岚3, 白玉白2, 樊玉国1   

  1. 1. 吉林大学超分子结构与材料教育部重点实验室;
    2. 吉林大学化学学院, 长春 130023;
    3. 云南大学单克隆抗体工程技术研究中心, 昆明 650091
  • 收稿日期:2003-01-13 出版日期:2003-05-24 发布日期:2003-05-24
  • 通讯作者: 徐蔚青(1957年出生),男.研究员,从事纳米材料及分子组装体光谱学研究.E-mail:wqxu@mail.jlu,edu.cn E-mail:wqxu@mail.jlu,edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29975011,20273022)

Immunological Identification with SERS-labeled Immunogold Nanoparticles by Silver Staining

XU Shu-Ping1, WANG Lian-Ying2, XU Wei-Qing1, ZHAO Bing1, YUAN Hang3, MA Lan3, BAI Yu-Bai2, FAN Yu-Guo1   

  1. 1. Key laboratory for Supramolecular Structure and Material of Minisry of Education of China ;
    2. College of Chemistry, Jilin University, Changchun 130023, China;
    3. Monoclonal Antibody Technology Center, Yunnan University, Kunming 650091, China
  • Received:2003-01-13 Online:2003-05-24 Published:2003-05-24

PDF (PC)

被引次数

43

摘要： 激光拉曼光谱技术近年来已成为研究生物分子结构常用的光谱手段.尤其在研究水溶液中蛋白质的结构和构象方面发挥了重要作用.然而,常规拉曼光谱的信号强度很低,限制了其在各个领域中的应用.表面增强拉曼光谱(SERS)和表面增强共振拉曼光谱(SERRS)技术可使信号增强6～10个数量级,尤其是SERS技术已发展到检测单分子的水平,更为其在生物方面的应用开拓了新的局.

关键词: SERS, 纳米粒子, 免疫, 标记

Abstract: Aspectral measurement combined with nanoparticle labeling and SERStechnique is described. The gold nanoparticles not only modified by the mouse monoclonal antibody agaist Hepatitis Bsurface antigen [IgG(H)] but also labeled by the p-mercaptobenzoic acid (MBA) were used as a probe. The immunogold nanoparticles specially react with the antibody against IgG(H)[anti IgG(H)] which was immobilzed on the quartz substrates. The identification between the immunogold colloids and the anti IgG(H) was examined by surface enhanced Raman specta(SERS) of MBA after and before silver staining and confirmed by UV-Vis absorption spectra.

Key words: Surface enhanced Raman spectroscopy(SERS), Nanoparticle, Immunization, Labeling

中图分类号: 

TrendMD: