高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (8): 1510.

• 研究论文 • 上一篇    下一篇

交河故城古车师人的线粒体DNA分析

崔银秋1,2, 段然慧2, 季朝能1, 朱泓2, 李惟2, 毛裕民1, 周慧2   

  1. 1. 复旦大学遗传工程国家重点实验室, 上海 200433;
    2. 吉林大学生命科学学院考古DNA实验室, 长春 130023
  • 收稿日期:2001-06-21 出版日期:2002-08-24 发布日期:2002-08-24
  • 通讯作者: Zhouhui@mail.jlu.edu.cn
  • 作者简介:周慧(1954年出生),女,博士,教授,博士生导师,从事分子生物学研究.E-mail:Zhouhui@mail.jlu.edu.cn
  • 基金资助:

    教育部科学技术重大项目;国家文物局边疆考古专项基金资助

Analysis of Mitochondrial DNA from the Ancient Ruins of Jiao-he

CUI Yin-Qiu1,2, DUAN Ran-Hui2, JI Chao-Neng1, ZHU Hong2, LI Wei2, MAO Yu-Min1, ZHOU Hui2   

  1. 1. State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China;
    2. Ancient DNA Laboratory, College of Life Science, Jilin University, Chang chun 130023, China
  • Received:2001-06-21 Online:2002-08-24 Published:2002-08-24

摘要: 从距今2000~2500年左右的交河故城古代人骨中提取古DNA,用4对重叠引物对线粒体基因组的调控区(363bp)进行了扩增及测序.线粒体基因组编码区的扩增片段用于限制性片段多样性分析.结果显示4个个体中具有3个DNA序列,其中来自不同墓穴的两个个体的序列相同,说明这两者间有密切的母系遗传关系.系统发育分析表明古车师的这4个个体分散分布在现代新疆维吾尔人的序列之中.从这些结果可以初步得出结论,即古车师人群并不是一个同源群体,在早期铁器时代,欧亚人群的混合就已经存在了.

关键词: 古DNA, 测序, 限制性片段多样性分析, 系统发育分析

Abstract: Mitochondrial DNA(MtDNA) was successfully extracted from four individual bones (femurs) from 2 tombs at the ancient Ruins of Jiao he in Turfan basin, dated about 2000~2500 years ago. Through four overlapping primers, we got nucleotide sequence of 363 bp length. Ancient MtDNAwas analyzed by restriction fragment length polymorphism(RFLP) analysis for the A, B, Cand Dhaplogroups and the sequencing of hypervariable region Ⅰ of the MtDNAcontrol region.The result shows that three haplotypes with 7 polymorphic sites in 4 individuals were obtained. Two specimens (male in each tomb) share the same haplotype. It is reasonable to assume that the close blood relationship existed between them. Phylogenetic analysis indicated that Jushi population were scattered among modern Xinjiang Uighuis and did not fall into specific clusters, so our preliminary data imply that Jushi population was not a genetically homogenous population and an ancient mingling of Euro Asian population had existed prior to the early iron age.

Key words: Ancient DNA, Sequence, Restriction fragment length polymorphism analysis, Phylogenetic analysis

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