高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (7): 1392.

• 研究快报 • 上一篇    下一篇

金电极表面植物原生质体的固定化

张红平1, 祁玉兰1, 严曼明1, 韦毅2, 林仲华3, 江志裕1   

  1. 1. 复旦大学化学系;
    2. 复旦大学生命科学院, 上海 200433;
    3. 厦门大学化学系, 固体表面物理化学国家重点实验室, 厦门 361005
  • 收稿日期:2001-08-20 出版日期:2002-07-24 发布日期:2002-07-24
  • 通讯作者: 江志裕(1940年出生),男,教授,博士生导师,从事电化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29773009和29833060)

Immobilization of Plant Protoplast on Gold Electrode Surface

ZHANG Hong-Ping1, QI Yu-Lan1, YAN Man-Ming1, WEI Yi2, LIN Zhong-Hua3, JIANG Zhi-Yu1   

  1. 1. Department of Chemistry;
    2. Academy of Life Science, Fudan University, Shanghai 200433, China;
    3. Department of Chemistry, State Key Laboratory for Physical Chemistry of the Solid Surface, Xiamen University, Xiamen 361005, ChinaLaborato
  • Received:2001-08-20 Online:2002-07-24 Published:2002-07-24

PDF (PC)

关键词: 聚电解质, 大麦细胞, 固定化

Abstract: Barley protoplast was immobilized on poly(dially-dimethylammonium) (PDADMAC) modified gold electrode via the electrostatic attraction between the positively charged amine groups of PDADMACand the negatively charged barley protoplast. STMimage reveals that PDADMAC/Au(111) surface displays a nano-organized dot(4-6 nm) array structure. The processes of the PDADMACadsorption and of the barley protoplast immobilization were monitored from the in situ QCMfrequency change. The effects of the adsorbed mass and of the density and viscosity of medium on the frequency change of QCMwere discussed, respectively. The QCM result shows that the thickness of the adsorbed PDADMAClayer is 2.4 nm.

Key words: Polyelectrolyte, Barley protoplast, Immobilization

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