高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (6): 1038.

• 研究快报 • 上一篇    下一篇

镉-钙黄绿素配合物在玻片表面的毛细流成环组装及其数值化荧光成象分析研究

黄承志, 李原芳   

  1. 西南师范大学环境化学研究所, 重庆 400715
  • 收稿日期:2002-01-25 出版日期:2002-06-24 发布日期:2002-06-24
  • 通讯作者: 黄承志(1965年生),男,博士,教授,博士生导师,从事超分子化学与生物化学分析研究.E-mail:chengzhi@swnu.edu.cn E-mail:chengzhi@swnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20175017);教育部高等学校骨干教师计划项目(2000-65);重庆市应用基础研究资助

Ordered Ring Assembly of Cadmium-Calcein Complex Directed Under the Capillary Flow on the Glass Slide Supports and Its Digitalized Fluorescence Image Analysis

HUANG Cheng-Zhi, LI Yuan-Fang   

  1. Institute of Environmental Chemistry, Southwest Normal University, Chongqing 400715, China
  • Received:2002-01-25 Online:2002-06-24 Published:2002-06-24

PDF (PC)

被引次数

5

关键词: 镉, 钙黄绿素, 毛细流, 荧光成象分析

Abstract: Afluorescent ordered ring assembly was observed under a fluorescence microscope, and the assembly image was captured by using a digital CCDcamera for analytical purposes. When a droplet of diluted solution of cadmium-calcein complex in 0.01 mol/Lhexahydropyridine was spotted onto the surface of dimethyl dichlorosilane modified glass slide, a symmetrical ordered ring was formed with an outer diameter of 1.10 mm and a ring line width of 0.048 mm. It was found that the fluorescent molecules on the ring line followed a Gaussian distribution, and with which a quantitative equation was established. For a 0.1 μLdroplet of the calcium calcein complex, the maximal fluorescence intensity of the ring is proportional to the content of calcium over the range of 3.0×10-15～1.0×10-13mol, and the limit of detection is 2.56×10-16mol. The proposed method has been successfully applied to the quantification of Cd2+ in water samples of Jialingjiang River.

Key words: Cadmium, Calcein, Capillary flow, Fluorescence image analysis

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