高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (2): 230.

• 研究论文 • 上一篇    下一篇

急性力竭运动对大鼠下丘脑氨基酸神经递质的影响

张东明1, 张佳民1, 马万云1, 陈瓞延1, 韩慧婉2, 舒鸿钧2, 刘国诠2   

  1. 1. 清华大学物理系, 原子分子纳米科学教育部重点实验室, 北京 100084;
    2. 中国科学院化学研究所, 北京 100080
  • 收稿日期:2000-11-11 出版日期:2002-02-24 发布日期:2002-02-24
  • 通讯作者: 马万云(1956年出生),女,教授,博士生导师,从事单分子探测研究.E-mail:mawy@lsad.tsinghua.edu.cn E-mail:mawy@lsad.tsinghua.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:29875012;30070209);中国科学院九五基础重大项目(批准号:KJ-951-A1-507)资助

Effect of Exhausting Exercise on Amino Acid Neurotransmitters in Hypothalamus of Rats

ZHANG Dong-Ming1, ZHANG Jia-Min1, MA Wan-Yun1, CHEN Die-Yan1, HAN Hui-Wan2, SHU Hong-Jun2, LIU Guo-Quan2   

  1. 1. Key Laboratory of Atomicand Molecular Nanosciencesof Educational Ministry, Departmentof Physics, Tsinghua University, Beijing 100084, China;
    2. Center of Molecular Science, Instituteof Chemistry, Chinese Academyof Sciences, Beijing 100080, China
  • Received:2000-11-11 Online:2002-02-24 Published:2002-02-24

摘要: 用激光诱导荧光检测的高效毛细管电泳结合微透析技术测定急性力竭运动前后的大鼠下丘脑区细胞外液中的氨基酸神经递质的含量.实验结果表明,急性力竭运动后大鼠下丘脑区的细胞外液中的6种氨基酸(精氨酸、甘氨酸、赖氨酸、谷氨酸、丙氨酸、γ-氨基丁酸)有统计意义的增加,这一结果显示了下丘脑区的氨基酸在应激过程中很可能起着重要的作用.

关键词: 微透析, 氨基酸神经递质, 运动

Abstract: A simple and effective coupling of microdialysis and capillary electrophoresis with laser induced fluorescence detection technique was applied to the analysis of amino acid neurotransmitters in hypothalamus of rat after an acute exhausting exercise. The separation of amino acids was achieved by using an uncoated fused silica capillary(57 cm×75 μm i.d.) with a buffer of 10 mmol/Ldisodium tetraborate at pH 10 and an applied voltage of 12.5 kV. The detection limit was 10-10 mol/Lfor each amino acid. It is sufficiently sensitive and rapid for the determination of amino acid in 5μL Microdialysate. In comparison to pre-exercise, a significant increase in the levels of 6 hypothalamic amino acids(arginine, glycine, lysine, glutamic acid, alanine, γ-amino-n-butyric-acid) after exercise was found. These results demonstrate that increase of metabolic amino acids in hypothalamus of rats can be induced by exhaustive exercise and suggesting that amino acid neurotransmitters may play functional roles in the central effects of exercise.

Key words: Microdialysis, Amino acid neurotransmitter, Exercise

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