高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (10): 1864.

• 研究论文 • 上一篇    下一篇

过氧化氢酶荧光分析法及其在海洋水生生物酶活力测定中的应用

毛玉霞1, 郑洪2, 郭祥群1, 陈荣3, 郑微云3   

  1. 1. 厦门大学化学系, 教育部现代分析科学重点实验室;
    2. 海洋学系;
    3. 环境科学研究中心;厦门 361005
  • 收稿日期:2001-06-13 出版日期:2002-10-24 发布日期:2002-10-24
  • 通讯作者: 郭祥群(1955年出生),女,博士,教授,从事光谱分析化学研究.
  • 基金资助:

    福建省自然科学基金(批准号:D9920001)资助

Studies on the Fluorometric Method for Determining Activity of Catalase and Its Application to Marine Biosamples

MAO Yu-Xia1, ZHENG Hong2, GUO Xiang-Qun1, CHEN Rong3, ZHENG Wei-Yun3   

  1. 1. Departm ent of Chemistry and the Key Laboratory of Analytical Science of MOE;
    2. Department of Oceanography;
    3. The Enviromental Science Research Center, Xiam en U niversity, Xiamen 361005, China
  • Received:2001-06-13 Online:2002-10-24 Published:2002-10-24

摘要: 建立了一种简便、灵敏的测定过氧化氢酶的方法.方法的线性范围为1.7×10-3~1.7×10-2U/mL,检测限(LD=3S0/S)为8.5×10-4U/mL.将其用于海洋生物样品中过氧化氢酶活力的测定,结果令人满意.

关键词: 过氧化氢酶测定, 喹啉, 荧光分析, Fenton反应, 生物样品

Abstract: A new promising method for determining activity of catalase and its application are presented. The measurement of catalase activity is based on the inhibition of catalase on Fenton reaction. Catalase catalyzes the dissociation of H2O2, one of the main reactants of Fenton reaction which yields ·OH stoichiometrically, and restrains the increment of the fluorescence intensity, produced as a result of quinoline hydroxylation. The decrease of fluorescence intensity was linearly related to the activity of catalase and the linear range covered from 1.7×10-3 U/mL to 1.7×10-2 U/mL. A detection limit of 8.5×10-4 U/mL catalase was obtained. This method has been applied to the detection of catalase in marine biosamples and satisfactory results were obtained.

Key words: Catalase determination, Quinoline, Fluorometry, Fenton reaction, Biosamples

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