高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (4): 683.

• 研究论文 • 上一篇    下一篇

脂肪酶在负离子化PET表面的单分子层自组装膜研究

林贤福, 何琳, 徐立恒, 吕德水, 陈志春   

  1. 浙江大学分析测试中心, 杭州 310027
  • 收稿日期:1999-12-24 出版日期:2001-04-24 发布日期:2001-04-24
  • 通讯作者: 林贤福(1963年出生),男,博士,教授,从事高分子化学与物理研究.

Studies on the Lipase Monolayer Film Self-assembly on the Nagatively Charged Poly(ethylene terephthalate) Substrate

LIN Xian-Fu, HE Lin, XU Li-Heng, LÜ De-Shui, CHEN Zhi-Chun   

  1. Center of Analysis and Measurement, Zhejiang University, Hangzhou 310027, China
  • Received:1999-12-24 Online:2001-04-24 Published:2001-04-24

摘要: 研究了脂肪酶分子在表面负离子化聚对苯二甲酸乙二醇酯(PET)薄膜表面的分子自组装,由AFM和FTIR-ATR等方法研究脂肪酶单分子层膜的表面结构;活力测定表明:脂肪酶/PET自组装单分子层膜的酶活力表现率约为2.0左右.

关键词: 脂肪酶, 分子自组装, PET, AFM, FTIR-ATR

Abstract: The PET-CO2- film was prepared and the lipase was assembled on the surface of the PET CO2- substrate. The structure of the surface and activity of lipase/PETmonolayer were studied by FTIR-ATRand AFMand other methods. As shown in the results, AFMimages of lipase/PETmonolayer indicated that the surface fluctuation is in the range of nanometer scale and the depth of monolayer is less than 15 nanometers. The performance activity of lipase/PETmonolayer self assembly film is above 2.0.

Key words: Lipase monolayer, Molecular self assembly, Poly(ethylene terephthalate), AFM, FTIR-ATR

中图分类号: 

TrendMD: