高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (4): 566.

• 研究简报 • 上一篇    下一篇

一种新型手性配体交换色谱键合固定相

龙远德, 王群标, 黄天宝   

  1. 中国科学院成都有机化学研究所, 成都 610041
  • 收稿日期:2000-02-21 出版日期:2001-04-24 发布日期:2001-04-24
  • 通讯作者: 黄天宝(1943年出生),男,研究员,从事色谱分析研究.

A Novel Bonded Stationary Phase for Chiral Ligand Exchange-Chromatography

LONG Yuan-De, WANG Qun-Biao, HUANG Tian-Bao   

  1. Chengdu Institute of Organic Chemistry, the Chinese Academy of Sciences, Chengdu 610041, China
  • Received:2000-02-21 Online:2001-04-24 Published:2001-04-24

关键词: 手性配体交换色谱, 手性固定相, 异喹啉衍生物, DL-氨基酸

Abstract: Anovel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding (S)-1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid prepared from L-Phe to YWG-80 silica gel via 3-glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL-amino acids were achieved on the CBSPby using an aqueous solution of 2 mmol/L N(C2H5)3, 2 mmol/L HAc and 0.2 mmol/L Cu(Ac)2 as the mobile phase with a flow rate 10 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL-amino acids on the CBSPwas found to be between 1.11 and 1.51. The elution order of Disomer before Lisomer on the CBSPwas observed for all the DL-amino acids resolved except DL-Val. For DL-Pro, DL-Val and DL-Leu the elution order through the CBSPwas different from that through the chiral ligand exchange phases prepared from L-Pro or L-hydroxyl proline with a five membered ring structure.

Key words: Chiral ligand exchange chromatography, Chiral stationary phase, Isoquinoline derivative, DL-amino acid

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