高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (2): 215.

• 研究简报 • 上一篇    下一篇

流动注射化学发光植物组织传感器测定草酸盐

彭友元1, 秦伟2, 章竹君2   

  1. 1. 泉州师范学院化学系, 泉州 362000;
    2. 陕西师范大学化学系, 西安 710062
  • 收稿日期:2000-01-21 出版日期:2001-02-24 发布日期:2001-02-24
  • 通讯作者: 彭友元(1975年出生),女,硕士,助教,从事化学发光分析研究.
  • 基金资助:

    国家自然科学基金(批准号:39730160)资助

Plant Tissue-based Chemiluminescence Flow Biosensor for the Determination of Oxalate

PENG You-Yuan1, QIN Wei2, ZHANG Zhu-Jun2   

  1. 1. Department of Chemistry, Quanzhou Normal College, Quanzhou 362000, China;
    2. Department of Chemistry, Shaanxi Normal University, Xi'an 710062, China
  • Received:2000-01-21 Online:2001-02-24 Published:2001-02-24

关键词: 组织传感器, 流动注射, 化学发光, 草酸盐

Abstract: Anovel chemiluminescence(CL) biosensor for the determination of oxalate is presented in this paper. It is based on the CLreaction between luminol, immobilized on an anion-exchange column, and hydrogen peroxide generated on-linevia oxalate by the catalytic effect of oxalate oxidase in spinach in the flow cell. The biosensor responds linearly to oxalate concentration in the range of 4.0×10-7 to 1.0×10-4 mol/L, and the detection limit is 2×10-7 mol/L. Acomplete analysis could be performed in 1 min with a relative standard deviation of 3.7% for 1.0×10-5 mol/Loxalate(n=7). The system is stable for over 200 times determinations and has been applied in urine samples.

Key words: Biosensor, Flow injection, Chemiluminescence, Oxalate

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