高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (2): 197.

• 研究论文 • 上一篇    下一篇

蒙脱土修饰碳纤维电极的制备及其应用于活体测定脑神经递质

杨丽菊, 彭图治   

  1. 浙江大学西溪校区化学系, 杭州 310028
  • 收稿日期:2000-01-02 出版日期:2001-02-24 发布日期:2001-02-24
  • 通讯作者: 彭图治(1946年出生),男,教授,博士生导师,主要从事电分析化学和生物分析化学研究.
  • 基金资助:

    浙江省自然科学基金;分析测试基金资助

Construction of Montmorillonite-modified Carbon Fiber Electrode and ItsIn-vivo Detection of Neurotransmitter

YANG Li-Ju, PENG Tu-Zhi   

  1. Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China
  • Received:2000-01-02 Online:2001-02-24 Published:2001-02-24

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被引次数

31

摘要： 制备了蒙脱土修饰碳纤维电极,研究了其对神经递质多巴胺(DA)及5羟色胺(5HT)的富集作用,以及对负电性的代谢产物3,4二羟基苯乙酸(DOPAC)、5羟吲哚乙酸(5HIAA)及脑内大量存在的抗坏血酸(AA)排斥性能.该电极具有很高的灵敏度、分辨率和抗干扰性,对DA的检测下限达16×10-8mol/L,优于传统的Nafion修饰电极,对5HT的检测下限为1×10-7mol/L.在动物活体分析中,使用该电极成功地检测了大鼠双侧颈总动脉结扎再灌损伤时,脑纹状体中神经递质DA浓度的变化.

关键词: 蒙脱土, 修饰电极, 活体测定, 神经递质

Abstract: The construction of montmorillonite-modified carbon fiber electrode is reported. The modified electrode shows a good sensitivity and selectivity due to its accumulation to cations and inhabitation to anions. The detection limit of DAand 5-HTare 16×10-8 and 1×10-7 mol/L, respectively, which are more sensitive than Nafion modified electrode.In-vivo voltammetry, the electrode has directly detected the dramatic variation of DAconcentration in a rat brain with decreasing blood supply, while it effectively inhabits the oxidation of electroactive compounds and some neurotransmitter metabolites, such as AA, DOPAC, 5-HIAA.

Key words: Montmorillonite, Modified electrode, In-vivo detection, Neurotransmitter

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