高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (1): 43.

• 研究简报 • 上一篇    下一篇

葡萄糖发酵液D-核糖含量的高效液相色谱分析

张津枫1, 王健刚2, 邓国才1, 杨四海1, 陈荣悌1   

  1. 1. 南开大学化学系, 天津 300071;
    2. 天津市红十字医院, 天津 300130
  • 收稿日期:1999-10-18 出版日期:2001-01-24 发布日期:2001-01-24
  • 通讯作者: 张津枫(1975年出生),男,硕士研究生.

High Performance Liquid Chromatography Analysis of D-Ribose Content in Glucose Zymotic Fluid

ZHANG Jin-Feng1, WANG Jian-Gang2, DENG Guo-Cai1, YANG Si-Hai1, CHEN Rong-Ti1   

  1. 1. Department of Chemistry, Nankai University, Tianjin 300071, China;
    2. Tianjin Red Cross Hospital, Tianjin 300130, China
  • Received:1999-10-18 Online:2001-01-24 Published:2001-01-24

PDF (PC)

被引次数

28

关键词: 高效液相色谱法, D-核糖, 葡萄糖

Abstract: The method has been put forward to analyze the content of D-ribose obtained from glucose zymotic fluid. D-ribose was determined by HPLC, on a Sugar PakⅠcolumn, by using a refractive index detector and a mobile phase of pure water. We have also studied the separation conditions of sugars. It has many advantages, such as rapid analysis, high sensitivity and high precision and good reproducibility. The recovery of D-ribose were 95.5%—104%, RSDwere less than 1.5%, the detection limit of D-Ribose was 50 ng.

Key words: High performance liquid chromatography, D-ribose, Glucose

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