高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (8): 1227.

• 论文 • 上一篇    下一篇

以Nitrosulfophenol S为化学模型物研究非氨基偶氮染料的致癌性

朱敏1, 韩树波1, 张书胜1, 袁倬斌1, 沈含熙2   

  1. 1. 中国科学技术大学研究生院, 北京 100039;
    2. 南开大学化学系, 天津 300071
  • 收稿日期:1999-09-02 出版日期:2000-08-24 发布日期:2000-08-24
  • 通讯作者: 袁倬斌(1938年出生),男,教授,博士生导师,从事电分析化学、毛细管电泳、扫描探针显微镜的研究.
  • 基金资助:

    国家自然科学青年基金(批准号:29905003)

Investigation of Carcinognic Non-aminoazo Dyes with Nitrosulfophenol S as Their Chemical Model

ZHU Min1, HAN Shu-Bo1, ZHANG Shu-Sheng1, YUAN Zhuo-Bin1, SHEN Han-Xi2   

  1. 1. Graduate School, USTC, Beijing 100039, China;
    2. Department of Chemistry, Nankai University, Tianjin 300071, China
  • Received:1999-09-02 Online:2000-08-24 Published:2000-08-24

摘要: 以Nitrosulfophenol S作为非氨基偶氮染料致癌性研究的化学模型物,用可见吸收光谱法、毛细管区带电泳法和电分析化学法等研究了该模型物在酶催化系统模型HRP/H2O2体系的作用下发生氧化断裂反应的过程,确定了反应的主要产物为致癌的重氮萘离子,并与DNA有效结合.讨论了断裂发生的酸度、温度及浓度条件.同时研究了体内多种生理性物质对断裂反应的抑制和激活影响.

关键词: 致癌物, Nitrosulfophenol S, 重氮萘离子, 过氧化物酶

Abstract: Nitrosulfophenol Sis advocated as the chemical model in the investigation of carcinogenic non-aminoazo dyes. Oxidative cleavage reaction of Nitrosulfophenol Sis carried out in the enzymic biotransform model-HRP/ H2O2 system, and the product, ultimate carcinogens aromicdiazonium ions, which bind to DNAis identified by visible spectra, capillary zone electrophoresis, electroanalytical chemical and other methods. The rate of oxidative cleavage depends on pH, temperature, and the concentrations of HRPand H2O2, then their optional oxidative conditions are discussed in detail. The inhibition and activation of various biological substances to the reaction is also investigated.

Key words: Carcinogen, Nitrosulfophenol S, Aromicdiazonium ions, Peroxidase.

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