高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (6): 873.

• 研究快报 • 上一篇    下一篇

电化学发光免疫传感器检测甲磺隆的研究

邢婉丽1, 晁福寰1, 蒋中华2, 马立人2   

  1. 1. 军事医学科学院卫生学环境医学研究所, 天津 300050;
    2. 军事医学科学院放射医学研究所, 北京 100850
  • 收稿日期:1999-10-15 出版日期:2000-06-24 发布日期:2000-06-24
  • 通讯作者: 邢婉丽(1968年出生),女,博士,主要从事生物传感器及生物芯片研究,现在清华大学生物芯片研究与开发中心工作.
  • 基金资助:

    国家博士后科学基金(批准号:6633);军事医学科学院创新基金

An Electrochemiluminescence Immunosensor for Determination of Methsulfuron-methyl

XING Wan-Li1, CHAO Fu-Huan1, JIANG Zhong-Hua2, MA Li-Ren2   

  1. 1. Institute of Hygiene and Environmental Medicine, Tianjin 300050, China;
    2. Institute of Radiation Medicine, Beijing 100850, China
  • Received:1999-10-15 Online:2000-06-24 Published:2000-06-24

关键词: 电化学发光, 免疫传感器, 甲磺隆

Abstract: An electrochemiluminescence(ECL) biosensor based on solid-phase antigen competitive immunoreactions was constructed to determine methsulfuron-methyl using Ru(bpy)32+ as labeling reagent. The coating hapten was bound to the surface of microparticles through avitin-biotin reaction. An electrochemical flow cell containing working electrodes and counter electrodes for initiation of the ECLreaction was constructed. Results showed that the detection limit for methsulfuron-methyl was 0.1 ng/mL.

Key words: Electrochemiluminescence, Immunosensor, Methsulfuron-methyl

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