高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (3): 363-367.

• 论文 • 上一篇    下一篇

药物扑热息痛分子模板聚合物的选择性富集与识别特性研究

郭洪声, 何锡文, 李一峻, 邓昌辉   

  1. 南开大学化学系, 吸附分离功能高分子材料国家重点实验室, 天津 300071
  • 收稿日期:1999-08-05 出版日期:2000-03-24 发布日期:2000-03-24
  • 通讯作者: 何锡文(1939年出生),男,教授,博士生导师,主要从事分子模板及分子识别研究.
  • 基金资助:

    国家自然科学基金(批准号:29775011);南开大学吸附分离功能高分子材料国家重点实验室资助

Studies of Selective Enrichment and Recognized Characteristics of Paracetamol Molecular Template Polymer

GUO Hong-Sheng, HE Xi-Wen, DENG Chang-Hui, LI Yi-Jun   

  1. Department of Chemstry, The Key Lab of Functional Polymer Materials for Adsorption and Separation, Nankai University, Tianjin 300071, China
  • Received:1999-08-05 Online:2000-03-24 Published:2000-03-24

摘要: 采用分子印迹技术合成了对药物扑热息痛有高选择性的模板聚合物.通过Scatchard分析研究了模板聚合物的结合特性;研究了模板聚合物的结合动力学.结果表明,以丙烯酰胺为功能单体的模板聚合物比以甲基丙烯酸为功能单体的聚合物具有更高的结合容量,且通过氢键作用形成一类等价的结合位点,其结合位点的离解常数为Kd=2.25×10-3mol/L.底物的选择性结合实验表明,与组成相同的非模板聚合物相比,扑热息痛分子模板聚合物对扑热息痛有较大的吸附富集性能和高度的选择性及识别能力.

关键词: 分子印迹技术, 模板聚合物, 扑热息痛, 底物选择性

Abstract: Amolecular templated synthetic polymer as the receptor highly selective for paracetamol was prepared by a molecular imprinting technique. The selective binding characteristics of the template polymer were evaluated by Scatchard analysis and Ultraviolet spectrometry. The results showed that the acrylamide as the functional monomer was more better than methacrylic acid for paracetamol selectivity, and a class of the same binding sites which could specially bind paracetamol by the interaction of hydrogen bonding were formed in the polymer and its dissociation constant was estimated to be 225×10-3 mol/L. The selective binding experiment for substrates indicated that the polymer gave a higher affinity and selectivity for paracetamol. It is possible to be a good adsorption and binding material in the selective enrichment and determination of trace paracetamol in complex biosamples.

Key words: Molecular imprinting technique, Template polymer, Paracetamol, Substrates selectivity

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