高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (2): 306.

• 论文 • 上一篇    下一篇

Cu(Ⅱ)-家蚕丝素蛋白质配合物的配位结构和高次结构

陈文兴1, 沈之荃2, 刘冠峰1, 白井汪芳3   

  1. 1. 浙江工程学院纤维分院, 杭州 310033;
    2. 浙江大学高分子系, 杭州 310027;
    3. 日本信州大学纤维学部, 上田 386-8567
  • 收稿日期:1999-10-27 出版日期:2000-02-24 发布日期:2000-02-24
  • 通讯作者: 沈之荃(1931年出生),女,教授,博士生导师,中国科学院院士,从事高分子化学研究.
  • 基金资助:

    教育部优秀年轻教师基金资助

Coordination Structure and Higher Order Structure of Cu(Ⅱ)-Silk Fibroin Protein(Bombyx Mori) Complexes

CHEN Wen-Xing1, SHEN Zhi-Quan2, LIU Guan-Feng1, SHIRAI Hirofusa3   

  1. 1. Collegeof Textile Science&Technology, Zhejiang Instituteof Science&Technology, Hangzhou 310033, China;
    2. Departmentof Polymer Science & Engineering, Zhejiang University, Hangzhou 310027, China;
    3. Facultyof Textile Science & Technology, Shinshu University, Ueda 386-8567, Japan
  • Received:1999-10-27 Online:2000-02-24 Published:2000-02-24

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被引次数

37

摘要： 家蚕丝素蛋白质在不同pH条件下经均相和不均相配位反应制备了Cu(Ⅱ)-丝素配合物,用可见光谱、电子自旋共振波谱(ESR)、X射线衍射(XRD)研究了其配位结构和高次结构.在碱性条件下(pH=10.60),丝素肽链主链的4个氮原子螯合Cu(Ⅱ)生成具有近似平面四方Cu(N)4结构的配合物;而在酸性条件下(pH=4.30,5.88),主要是丝素肽链的侧(端)基羧酸根键合Cu(Ⅱ)生成Cu(Ⅱ)(-COO-)(H2O)3和Cu(Ⅱ)(-COO-)2型配合物.讨论和描述了不同条件下生成的Cu(Ⅱ)-丝素配合物的高次结构.

关键词: 丝素蛋白质, 铜(Ⅱ)配合物, 配位结构, 高次结构

Abstract: The copper(Ⅱ) complexes of silk fibroin protein of Bombyx mori are prepared by homogeneous and heterogeneous phase coordination reaction at different pHvalues, and their coordination structures and aggregated structures are investigated by means of spectroscopy, electron spin resonance(ESR) and X-ray diffraction(XRD) measurements. Under the basic condition (pH=10.60), one type of complex is formed which contains four nitrogens from peptide main chain at the corners of a square in which the copper(Ⅱ) occupies the center, and while under the acid condition(pH=4.30, 5.88), other complex is obtained, which is mainly formed by the carboxyl of peptide side or end groups binding copper(Ⅱ) as Cu(Ⅱ)· (-COO-) (H2O)3and Cu(Ⅱ)(-COO-)2.The higher order structures of Cu(Ⅱ)-silk fibroin protein complexes prepared under various conditions are also discussed and described.

Key words: Silk fibroin protein, Copper(Ⅱ) complex, Coordination structure, Higher order structure

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