高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (2): 218.

• 研究简报 • 上一篇    下一篇

傅里叶变换红外光谱法应用于水-乙醇溶液中海南霉素的测定

邱江, 丁乐, 洪叶勤, 张嗣良   

  1. 华东理工大学生物反应器工程国家重点实验室, 上海 200237
  • 收稿日期:1998-02-24 出版日期:1999-02-24 发布日期:1999-02-24
  • 通讯作者: 邱江,男,33岁,博士.
  • 作者简介:邱江,男,33岁,博士.

Determination of Hainanmycin in the Aqueousalcohol Solution by Fourier Transform Infrared Spectroscopy

QIU Jiang, DING Le-Hong, YE Qin, ZHANG Si-Liang   

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai, 200237
  • Received:1998-02-24 Online:1999-02-24 Published:1999-02-24

摘要: 随着化学计量学方法处理光谱重叠和光谱采样技术的发展,红外光谱应用于多组份同时定量分析和过程分析的研究日益受到人们的关注[1~5].海南霉素(C47H79O15Na,分子量907.122)是国家一类新药,用于防治鸡球虫病,易溶于有机溶剂,不溶于水.

关键词: 傅里叶变换红外光谱, 衰减全反射, 偏最小二乘法, 定量分析, 有机相

Abstract: Fourier transform infrared(FTIR) spectroscopy equipped with horizontal attenuated total reflection(ATR) accessory was applied to the multicomponent quantitative analysis in organic phase. Anovel method was developed to directly determine the Hainanmycin(HNMS, an insoluble antibiotic from the fermentation) in water and ethyl alcohol solution. Calibration models were established on the basis of partial least squares with infrared spectra(IR) and secondderivative absorbance spectra(DIR) of the standard mixtures. The better predicted results is obtained especially at lower concentration of HNMS by DIR method. The standard errors of prediction of HNMS by IRor DIRare 0.18 and 0.19 g/L, respctively. The standard errors of prediction of water and alcohol are 0.0011 volume ratio by either quantitative method. The method is rapid and convenient without any pretreatment.

Key words: Fourier transform infrared spectroscopy, Attenuated total reflection, Partial least squares, Quantitative analysis, Organic phase

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